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. Author manuscript; available in PMC: 2015 Jun 4.
Published in final edited form as: Traffic. 2000 Dec;1(12):941–951.

Table 1.

Purification of AL pores

Fraction Protein (mg) Yield (%) Approximate fold purification
Total extract 1 100 100 1
I Crude membrane 109 80 8
II Flotation 35 75 24
III Velocity gradient 26 70 29
IV Detergent extract 1.4 55 432

Pores were purified as described in Materials and Methods, and the fold-enrichment was determined by densitometric scanning of western blots of the fractions, probing for the nucleoporin Nup93. Fractions I–IV correspond to those in Figure 1C.