Protein Analysis of Bronchoalveolar Lavage Fluid at an aspirated PM dose of 50 μg. Comparison of (A) Total protein leakage analysis, (B) cell differentiation, and (C) cell counts in BALF 24 hr post-single aspiration of PM2.5 (50 μg = 1 mg/ml) from either JC, ZH, or ZH PM2.5 spiked with all of the metals and metalloids found in the JC PM sample: AsO3, SeCl4, CuSO4, and NiSO4 (n = 6/group), positive controls: NiSO4 + H2O (n = 4), NiSO4 + Carbon (n = 4) and water as a negative controls (n = 10; *p < 0.05; **p < 0.01; ***p < 0.001).