Table 2.
Number of animals per exposure group, duration, and biological assessments
| Exposure time | N | PM sample | PM nominal concentration | Collected tissues/biological fluids | Biological assessment |
|---|---|---|---|---|---|
| 1x | 6 | JC | 0.5 μg | BALF | BALF evaluation of cell differentials, counts, and protein concentration |
| 1x | ZH | ||||
| 1x | ZH + NiSO4 | ||||
| 1x | ZH + CuSO4 | ||||
| 1x | ZH + AsO3 | ||||
| 1x | ZH + SeCl4 | ||||
| 1x | 4 | NiSO4 + H2O | |||
| 1x | NiSO4 + Carbon | ||||
| 1x | 8 | JC | 0.25 μg | BALF, Blood | BALF evaluation of cell differentials, counts, and protein, various inflammation and vascular dysfunction, vessel function |
| 3 wks | 14 | BALF, Blood, Mesenteric Artery | |||
| 2x/wk | |||||
| 1x | 8 | ZH | BALF, Blood | ||
| 3 wks | 8 | BALF, Blood, Mesenteric Artery | |||
| 2x/wk | |||||
| 1x | 8 | ZH + NiSO4 | BALF, Blood | ||
| 3 wks | 16 | BALF, Blood, Mesenteric Artery | |||
| 2x/wk |
H2O controls were matched to each exposure group.