Table 1.
Nomenclature, amino acid sequence, and phase behavior of expressed proteins
Protein Label |
*Amino Acid Sequence | M.W. | ****Phase Behavior | ||
---|---|---|---|---|---|
**Exp [kDa] |
***Obs [kDa] |
Intercept, b [°C] |
Slope, m [Log10(µM)]−1 |
||
V96 | G(VPGVG)96Y | 39.6 | 39.5 | 36.1 | 3.25 |
LV96 | GEDASSDSTGADPAQEAGTSKPNEEISGPAEPASPPETTTTAQET SAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGG VPGGKQFIENGSEFAQKLLKKFSLLKPWAGLVPRGSG(VPGVG)96Y |
52.5 | 52.3 | 28.6 | 1.19 |
Lacrt | GEDASSDSTGADPAQEAGTSKPNEEISGPAEPASPPETTTTAQET SAAAVQGTAKVTSSRQELNPLKSIVEKSILLTEQALAKAGKGMHGG VPGGKQFIENGSEFAQKLLKKFSLLKPWAGLVPR |
12.8 | 12.7 | NA | NA |
After the start codon, a glycine spacer was added during cloning, which is not present on human Lacrt
Expected M.W. was estimated for each sequence, which excludes the methionine start codon.
Observed M.W. was measured by MALDI-TOF
ELPs in PBS phase separate above a line with slope m and intercept b as defined by Eq. 1. Not applicable (NA).