Table 1. TPP for point-of-care diagnosis for patients in the acute phase of Chagas disease.

Needs for Diagnosis	Medical Conduct	Samples and Sampling	Infrastructure	Technical Skills	Testing Site, Turnaround Time	Reading	Taxonomic Diagnosis	Sensitivity	Specificity
Congenital transmission	Serodiagnosis of pregnant women and women admitted at delivery living or born in endemic countries (knowing that >70% have no signs or symptoms)	Samples processed individually. (i) Maximum. 2 ml of cord or peripheral blood obtained specifically for diagnostic test; (ii) Blood sample collected for routine screening for infectious or metabolic diseases; (iii) Ideal: urine sample	(i) Ideal: processing at point of care; (ii) Less desirable: samples processed in a reference laboratory transported without cold chain	Good laboratory practices (GLP)–trained technical staff with quality certification. Screening conducted by staff who assisted the childbirth	Primary health centre (PHC), hospital or delivery institution. Ideal timing: <1 h, up to a maximum of 12 h from sampling	Qualitative	Single universal test should detect all circulating strains	>95%	100%. Ideal: integrated into routine health care screening (e.g., metabolic screening)
Vector and oral transmission	(i) Differential diagnosis for at risk population with febrile syndrome; (ii) Active search in cases of possible exposure (contacts)	Samples processed individually. (i) 2–5 mL blood or serum; (ii) Ideal: urine, saliva sample	(i) Ideal: processing at point of care; (ii) Less desirable: samples processed in a reference lab and transported without cold chain	GLP-trained technical staff with quality certification	PHC and/or community-based diagnosis facility. Ideal timing: <1 h from sampling	Qualitative/quantitative	Single universal test should detect all circulating strains	>95%	100%. Ideal: integrated into routine health care screening (e.g., metabolic screening). Should differentiate T. cruzi from T. rangeli
Reactivation of infection associated with immune suppression in organ transplants. Blood transfusion transmission	Active surveillance	Samples processed individually. Blood, cerebral spinal fluid, tissue from chagoma	(i) Ideal: processing at point-of-care; (ii) Less desirable: samples processed in a reference laboratory and transported without cold chain	GLP-trained technical staff with quality certification	Reference medical facility, blood banks, and hospital. Ideal timing: <1 h from sampling	Qualitative/quantitative	Single universal test should detect all circulating strains	>95%	100%. Ideal: integrated into routine screening. Should differentiate between T. cruzi and T. rangeli. In the case of diagnosis in central nervous system manifestations of HIV/AIDS, it should differentiate T. cruzi from other opportunistic infections, such as toxoplasmosis.