Table 2. TPP for point-of-care diagnosis for patients in chronic phase of Chagas disease.

Needs for Diagnosis	Medical Conduct	Samples and Sampling	Infrastructure	Technical Skills	Testing Site, Turnaround Time	Reading	Taxonomic Diagnosis	Sensitivity	Specificity
Asymptomatic infected patients, referred symptomatic individuals, and positive blood donors	Active search in endemic/nonendemic and remote areas; prenatal screening	Samples processed individually. Ideal: saliva/urine; Alternative: whole blood, plasma or serum	Point of care, including community-based facility external to health center (no transportation required)	Adequately trained technical staff or community works with minimum quality certification standards	PHC and community setting (home, school, or community center); Ideal timing: <1 h from sampling	Qualitative	Single universal test should detect all circulating strains	Equal to or greater than standard serological tests	100%. No cross-reaction with other parasites (e.g., Leishmania, T. rangeli)