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. 2015 Jun 4;10(6):e0128870. doi: 10.1371/journal.pone.0128870

Fig 1. Elevation of extracellular NaCl increases expression of pro-inflammatory mediators in endothelial cells.

Fig 1

HUVECs were exposed to media in which NaCl was elevated for 4 days from 270 mosmol/kg to the total osmolality indicated in the figure panels. mRNA for 84 genes was measured by real-time PCR as described in Methods. (A) mRNA of adhesion molecules VCAM-1, E-selectin and of chemokine MCP-1 showed biggest increase upon exposure to high NaCl. The graph plots fold changes relative to 270 mosmol/kg of mRNA for 84 genes related to endothelial cell biology. Each dot shows mean value from 3 independent experiments. (B) Statistical analysis for VCAM-1, E-selectin and MCP-1 (mean ±SEM, *P<0.05, N = 3)