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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Mol Cancer Ther. 2015 Feb 11;14(3):779–787. doi: 10.1158/1535-7163.MCT-14-0228

Figure 4. Notch- cells fails to generate Notch+ cells in vitro.

Figure 4

(A) Notch reporter lentivirus infected Sum159 cells were presorted by FACS and Notch+ cells were kept in culture for two weeks during which Notch+ cells generated Notch+ as well as Notch- cells. (B) Using the presorted Notch+ cells, single Notch+ or Notch- cells were FACS sorted and independent clones were grown. We analyzed these independent clones that are generated from single Notch- (n=13) or Notch+ (n=12) cells. None of the clones of single Notch- cells was able to generate the Notch+ cell population while the clones generated from single Notch+ cells were able to generate both Notch+ as well as Notch- cell populations. Means ± SD (n=3),*p≤0.05, ** p≤0.005.