Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2015 May 4;22(6):492–498. doi: 10.1038/nsmb.3015

Figure 5. Pore loop residues are required for disassembly.

Figure 5

(a) Surface presentation of the Vps4 hexamer model based on the p97 hexameric structure (PDBID: 1E32 (34)). Residues 206 and 207 of one of the protomers were colored yellow and magenta, respectively, to highlight their locations in the pore. (b) SDS-PAGE gel of Vps4W206A M207A-mediated disassembly of Vps24-2 filament. (c) Quantification of Vps4WT, Vps4W206A M207A and Vps4CF mediated disassembly of Vps24-2 filament by sedimentation. Error bars are the s. d. of three measurements.