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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Leukemia. 2015 Feb 4;29(6):1290–1300. doi: 10.1038/leu.2015.18

Figure 3.

Figure 3

Shp2E76K induces cytokine hypersensitivity in MLL-AF9 leukemic cells. (A) Lin-c-kit+ bone marrow cells were transduced with the indicated retroviruses and selected. Cells were grown in liquid culture and counted every other day. The total number of cells is plotted. (B) Shp2 expression in MA9+MIEG3, MA9+E76K cell lines are detected by western blot following whole cell lysis. (C) MA9+MIEG3 and MA9+E76K cells were cultured in serially diluted IL3 for 3 days. The growth rate was calculated and plotted showing cytokine hypersensitivity by MA9+E76K cells. (* p<0.0001; 2-way Anova; biological duplicates). (D) Western blotting was performed for total Erk and p-Erk in MA9+MIEG3 and MA9+E76K cells. Cells were cultured in serum free OPTI-MEM media overnight before a pulse with different doses of IL3 for 10 minutes.