Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Leukemia. 2015 Feb 4;29(6):1290–1300. doi: 10.1038/leu.2015.18

Figure 5.

Figure 5

Mcl1 partially phenocopies Shp2E76K in MLL-AF9 cells. (A) Hematopoietic progenitor cells were transduced with empty vectors, Mcl1 alone, MA9 alone or MA9+Mcl1 and plated in methylcellulose. Colonies were counted after 7 days and replated for a total of three rounds. Colonies numbers are shown relative to the highest number of colonies observed. Error bars indicate standard deviation of biological duplicate experiments. (* p<0.05; t-test). Plates were stained with INT and imaged with a ChemiDoc XRS+ Imaging System after the 3rd round. Data represents average of 3 independent experiments. (B) MA9+MSCVpuro and MA9+Mcl1 cells were cultured in serially diluted doses of IL3. Cells were counted after three days and growth rates are plotted. Data shows cytokine hypersensitivity with the overexpression of Mcl1. (* p<0.0001; 2-way Anova; biological duplicates). (C) Limiting dilution colony formation assays were performed with MA9+MIEG3 and MA9+E76K cells. The average number of colonies from biological duplicate experiments after 7 days in culture is plotted against seeding cell number with the dashed line representing a 95% confidence interval. CFU potential is determined by 1/slope. (D) Mcl1 overexpression was detected by western blotting.