Fig 6. Blood-brain barrier permeability of AP-12 at the dose 1 mg/kg in C57BL/6J mice.

(A) AP-12 detected in the whole brain homogenate by mass spectrometry (LCMS-MRM) analysis with the peak corresponding to AP-12 eluted from the column at 2.68 min. (B) AP-12 spiked into untreated mouse brain homogenate at 0.1 μg/ml and extracted. (C) AP-12 standard curve from 1.0 to 100 ng/ml (r = 0.9989).