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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Feb 20;13(5):828–838. doi: 10.1158/1541-7786.MCR-15-0016-T

Figure 1. LncRNA, DRAIC is downregulated in CR cells and decreased by R188.

Figure 1

(A) Left: analysis of published RNA-seq datasets. Right: Relative expression (FPKM: Fragments Per Kilobase of exon per Million fragments mapped) of LOC728431 and LOC145837 (DRAIC) in LNCap cells treated with vehicle or R1881 and C4-2B cells treated with vehicle (B) Top and bottom show LOC728431 and LOC145837 (DRAIC) gene structures and qPCR primer locations, respectively. (C) The expression of LOC728431 in a panel of prostate cancer cells cultured in the growth medium was measured by RT-qPCR and normalized to GAPDH. Mean±S.D. n=3. The expression in LNCap is set as 1. (D) LNCap cells were treated with R1881 at different doses (left) and times (right) and the expression of LOC728431 was measured by RT-qPCR. The expression in 0 pM or 0 h is set as 1. Rest as in Fig. 1C. (E) The expression of DRAIC in a panel of prostate cancer cells cultured in growth medium was measured by RT-qPCR. Rest as in Fig. 1C. (F) LNCap cells were treated with R1881 at different doses (left) and times (right) and the expression of DRAIC was measured by RT-qPCR. Rest as in Fig. 1C, D.