Table 2. Topology of mouse OC-STAMP.
| Feature | Orientation | aa #’s | Sequence |
|---|---|---|---|
| Intracellular loop 1 | inside | 1–24 | MRTIRAATEHLFGLGWKFWRLGIC |
| Transmembrane helix 1 | in to out | 25–47 | KAVVPLQAAWKAFSQPVPASCNE |
| Extracellular loop 1 | outside | 48–51 | LLTQ |
| Transmembrane helix 2 | out to in | 52–74 | LLLCVSLASLIAGLAHHWLVSLQ |
| Intracellular loop 2 | inside | 75–86 | LYPLGPPALVTS |
| Transmembrane helix 3 | in to out | 87–109 | LCGLFVFLSLGLVPPIRCLFVLS |
| Extracellular loop 2 | outside | 110–227 | VPTLGSKQGRRLLLSYSAANLAVAVVPNVLGNVRAAGQVLSCVTEGSLESLLN̳TTYQLRQAARELGPASRAGSRSLTFEVEGKGSAFRLHMHTITQEILEDFSGLEFLARAALGTQRV |
| Transmembrane helix 4 | out to in | 228–250 | VTGLFLLGLLGESAWYLHRYLTD |
| Intracellular loop 3 | inside | 251–300 | LRFDNIYATRQLVRQLAQAGATHLLTSPPPWLLQTAQPKLSREELLSCLL |
| Transmembrane helix 5 | in to out | 301–323 | RLGLLALLLVATAVTVASDYGAF |
| Extracellular loop 3 | outside | 324–398 | LLAQAAVAWAQKLPTVPITLTVKYDASYKVLDFILFVLNQPPVESVFASMQRSFQWELRFTPHDCHLPQAQPPRV |
| Transmembrane helix 6 | out to in | 399–421 | TAALAAGALQLLAGATLVLQAYA |
| Intracellular loop 4 | inside | 422–498 | WRLRHTIAASFFPDQEARRLSHLQARLQRRHNQSDHLNKQPGTMATRESRKPGQGTRTLESQGPQAHDSLGPPYDLE |
Transmembrane orientation of mouse OC-STAMP (GenBank accession # NP_083297.1) predicted by consensus of 5 algorithms utilized by the TOPCONS server (http://topcons.cbr.su.se/: SCAMPI-seq, SCAMPI-msa, PRODIV, PRO, and OCTOPUS). Also, we have shown in this report that the N- and C-termini are intracellular and that N162 (bold, underlined) is glycosylated, supporting those predictions.