Fig. 4.

Case vs. control cell–substrate adhesion. (a) The hiPSC-RPE ECIS adhesion assay: comparing the resistances of the case and control cell lines during the first 24 h of culture, showed how the case cell line attached more quickly to the electrodes and were able to reach a plateau faster than the control cell line. (b) Attachment slope: the slopes of the attachment curves for both case and control cell lines were calculated through a linear fit. The slope was significantly higher for the case cell line (P<0.01) which was consistent with the case cells arriving at a plateau before the control cells. (c) Cell–substrate adhesion parameter (α): ECIS parameter (alpha) of the case cell line was significantly higher than that of the control cell line (P=0.002), indicating that the case cell line obtained a higher cell–substrate adhesion. (d) Adhesion biochemical assay: the case cell line showed stronger adhesion to various ECM proteins than the control cell line.