FIG 4.

NOS1AP isoforms containing the PTB domain interact with Scribble. (A) Endogenous NOS1AP isoforms were precipitated (I.P.) from rat brain lysate with the indicated antibodies. Bound complexes were analyzed by immunoblotting using anti-Scribble, anti-nNOS, and pep-NOS1APc antibodies, as indicated at the bottom. (B and C) cDNA constructs encoding the indicated proteins were transfected into HEK293T cells. (B) Bound complexes were analyzed with anti-myc and anti-GFP antibodies. (C) Whole-cell lysates with input levels.