FIG 3.

NF90 Tg mice do not display an accumulation of centronuclear myofibers. (A) H&E staining of the cross-sectional area of quadriceps from WT and NF90 Tg mice at 15 weeks of age. Bars, 50 μm. (B) Numbers of centronuclear quadriceps fibers in WT and NF90Tg mice at 15 weeks of age. One hundred quadriceps fibers from each mouse were examined. Data are expressed as means ± standard deviations (n = 3). Statistical significance was calculated by using two-tailed Student's t test. n.s., not significant. (C) Myogenin and p21 expression levels in the skeletal muscle of WT and NF90 Tg mice at 14 to 21 weeks of age were measured by qRT-PCR. Hypoxanthine phosphoribosyltransferase was used as an internal control. Data are expressed as means ± standard deviations (n = 4 to 6). Statistical significance was calculated by using two-tailed Student's t test. **, P < 0.01 compared to the WT.