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. 2015 Jun 4;35(13):2295–2308. doi: 10.1128/MCB.01297-14

FIG 8.

FIG 8

Dnm2 protein levels are elevated in the skeletal muscles of NF90-NF45 dbTg mice. (A) Dnm2 mRNA expression in the skeletal muscles of WT (n = 7) and dbTg1 (n = 7) mice was analyzed by qRT-PCR. Hypoxanthine phosphoribosyltransferase (HPRT) was used as an internal control. Data are expressed as means ± standard deviations. Statistical significance was calculated by using two-tailed Student's t test. n.s., not significant. (B) Western blot analysis of Dnm2 levels in tissue extracts of skeletal muscles from WT and dbTg1 mice. GAPDH was used as a loading control. (C) Intensities of specific bands in the Western blot analysis shown in panel B were measured with a densitometer and are presented as a graph. Each dot and bar indicate one experiment and an average, respectively. *, P < 0.05 compared to the WT, determined by two-tailed Student's t test. (D) Immunohistochemical analysis of Dnm2 in quadriceps from WT and dbTg1 mice at 15 weeks of age. Bars, 50 μm.