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. 2015 Jun 4;35(13):2356–2365. doi: 10.1128/MCB.00150-15

FIG 2.

FIG 2

Systemic Map4k4 deletion reduces adipose tissue inflammation without changing adiposity. Animals were fed an HFD for 16 weeks, and visceral adipose tissue was isolated. (A) Relative levels of mRNA expression of various inflammatory genes as indicated (n = 7). (B) The VAT stromal vascular fraction was isolated and fluorescence-activated cell sorter (FACS) analysis performed. Representative FACS scatter plots for control mice and iMap4k4-KO mice are shown. The upper plots show F4/80- and CD11b-positive cells, while the lower plots show CD11b- and CD11c-positive cells (n = 10 to 13). (C) Number of macrophages (F4/80-positive cells) per milligram of VAT (n = 10 to 13). (D) Percentage of CD11c-positive cells (n = 10 to 13). (E) CD11c-positive cells per milligram of VAT (n = 10 to 13). Data represent average means ± SEM (*, P < 0.05; **, P < 0.005).