FIG 2.

Genetic inhibition of rictor or Sin1 but not raptor expression (A to D) and enforced expression of ectopic rictor but not raptor (E) alter Mcl-1 protein levels (A to E) but not its mRNA level (F). (A to E) Whole-cell protein lysates were prepared from the indicated transfectants or MEFs (A, C, and D), A549 cells 48 h after transfection with the indicated siRNAs (B), and 293T cells 48 h after transfection with plasmids harboring a given gene (E). Lysates were then subjected to Western blotting to detect the indicated proteins. SE, short exposure. (F) Total cellular RNA was prepared from the indicated MEFs and then subjected to RT-PCR for detection of Mcl-1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs. Ctrl, control.