FIG 4.

Analysis of μ and γ3 heavy chain transcripts in A150 pro-B and pre-B cells. Pro-B and pre-B cells were sorted from the bone marrows of WT and A150 mice, and RT-PCR was performed on total RNA. A specific primer that binds the leader sequence of the inserted V_H segment (schematics on the left), degenerate primers that bind the V_H7183 or V_HJ558 gene family, and specific Cμ1 or Cγ3-1 reverse primers were used to amplify the mature VDJ-Cμ and VDJ-Cγ3 transcripts. The actin transcripts were used for normalization of single-stranded DNA input. Single-stranded cDNAs from Rag2^−/− and A150/A150 Rag2^−/− mice were included as controls. The corresponding histograms are shown (n = 2 for each experiment, with a pool of >3 mice). ***, P < 0.001.