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Effects of Tip110 expression on p53 and HIF-1α under hypoxia. (A to D) U2OS (A) and Saos-2 (C) cells were transfected with a Tip110-His or cDNA3 plasmid for 24 h and cultured under 21% or 1% O2 for 24 h. Cells were harvested and prepared for Tip110, p53, HIF-1α, and p300 protein expression by Western blotting. Levels of Tip110, HIF-1α, p300, and p53 protein expression were quantitated using β-actin as a reference (B and D). The data are representative of three independent experiments.
