Figure 5. ISW1a remodeling is blocked by Lac repressor, while SWI/SNF remodeling evicts Lac repressor from the template.
Shaded regions indicate locations of Lac repressor binding sequence and 601NPE. (A) Distributions of locations of nucleosomes before remodeling (upper plot), after remodeling by ISW1a without Lac repressor (middle plot), and after remodeling by ISW1a with Lac repressor (lower plot). Lac repressor binding sequence was separated from the 601NPE by 50 bp. Nucleosomes remodeling was carried out in 1 nM ISW1a with 1 mM ATP for 10 min with or without Lac repressor. (B) Representative traces in the case of before SWI/SNF remodeling (top plot; N = 25) and after remodeling (middle and bottom plots; N = 27). Lac repressor binding site was separated from the 601NPE by 10 bp. Nucleosomes were remodeled by 1.5 nM SWI/SNF with 1 mM ATP for 10 min. The middle plot shows an example trace where a nucleosome was remodeled to the other side of the Lac repressor and the bottom plot shows an example trace where a nucleosome was remodeled to the same side of Lac repressor.