FIGURE 5.
The RacRS system is important for the generation of glutamate out of glutamine. Growth curves were generated of the wt 81116 (A), the racR (B), and ggt (C) mutants grown in DMEM (circles) or with the addition of 10 mM glutamate (squares) or 10 mM glutamine (triangles) at 1% O2 with the addition of 10 mM TMAO as electron acceptor. (D) The same as (C) but with the extra addition of 10 mM aspartate to the medium. Data represent the mean and standard error of three independent experiments. Significance was calculated using the maximally obtained OD600nm values. ns, not significant; *p < 0.05, ***p < 0.001.