Table 1.
Bacterial strains and plasmids used in this study.
| Bacterial strains and plasmids | Relevant characteristics | Origin or reference |
| Strains | ||
| Escherichia coli TG1 | supE hsdΔ5 thiΔ(lac-proAB) F’ (traD36 proAB+ lacIq lacZΔM15); used for general cloning | Green and Sambrook (2012) |
| E. coli BL21(DE3) | F- ompT hsdSB (rB- mB-) gal dcm (DE3); used for protein overexpression | Novagen |
| E. coli S17 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 (Tmpr Strr); used for conjugation | Parke (1990) |
| Campylobacter jejuni 81116 | Wildtype | Palmer et al. (1983) |
| C. jejuni ggt | 81116 derivative ggt::Cm | This study |
| C. jejuni racR | 81116 derivative racR::Cm | van der Stel et al. (2015) |
| C. jejuni racS | 81116 derivative racS::Cm | This study |
| C. jejuni racRS | 81116 derivative racRS::Cm | This study |
| C. jejuni racR+p | 81116 derivative racR::Cm + pMA1-1261-1263 | This study |
| C. jejuni racRS+p | 81116 derivative racRS::Cm + pMA1-1261-1263 | This study |
| C. jejuni 11168 | Wildtype | Palmer et al. (1983) |
| C. jejuni 81-176 ggt | 81-176 derivative ggt::Cm | This study |
| Plasmids | ||
| pBluescript II KS | ApR; 3.0 kb; LacZα | Stratagene |
| pGEM-T Easy | ApR; 3.0 kb; LacZα; TA cloning vector | Promega |
| pT7.7 | ApR; 2.5 kb; expression vector | Tabor and Richardson (1985) |
| pMA1 | KmR; 10 kb; E. coli/C. jejuni shuttle vector | van Mourik et al. (2008) |
| Plasmids constructed for mutagenesis | ||
| pUWM799 | ApR; 4.2 kb; pBluescript II KS/internal fragment of C. jejuni ggt | This study |
| pUWM804 | ApR CmR; 4.9 kb; pBluescript II KS/ggt::Cm | This study |
| pGEM-1261-1263 | ApR; 5.5 kb; pGEM-T Easy/C. jejuni 1261-1263 | This study |
| pGEM1261::Cm | ApR CmR; 6.3 kb; pGEM-T Easy/racR::Cm | This study |
| pGEM1262::Cm | ApR CmR; 6.3 kb; pGEM-T Easy/racS::Cm | This study |
| pGEM1261-62::Cm | ApR CmR; 5.3 kb; pGEM-T Easy/racRS::Cm | This study |
| Plasmids constructed for complementation | ||
| pMA1-1261-1263 | KmR;12.5 kb; pMA1/Cj1261-1263 | van der Stel et al. (2015) |