FIGURE 5.

Effect of vATPase and neuraminidase inhibition on viral infection and shedding in bronchial explants. Tissue explants were treated with (A) oseltamivir (100 nM) or (B) TVB024 (5 μM) for 2 h prior to infection with influenza virus and then incubated for a total of 48 h. Cells dispersed from the tissues were analyzed by flow cytometry for epithelial influenza infection, and culture media were assessed for viral protein immunoreactivity, as previously described. Data shown are mean ± SEM of five experiments. *p < 0.05. INF, infected; NI, not infected.