Up- and Downregulation of SDG711 Affected Panicle Development.
(A) Panicle phenotypes of SDG711 overexpression (OX), gain-of-function mutant (sdg711), and RNAi plants compared with the wild type (WT). Means ± sd of primary branches and spikelets per panicle (n = 30) are presented on the right. Significant differences between the transgenic or mutant plants and the wild type (Student’s t tests, P value < 0.01) are marked by double asterisks.
(B) Longitudinal section of inflorescence meristem from the indicated genotypes.
(C) In situ hybridization of SDG711 transcripts at different developmental stages of the inflorescence meristem. Length of the meristem was ∼1 mm in (a), 0.25 mm in (b), 0.5 mm in (c), 1.5 mm in (d), and 3 mm in (e). Probe used was sense in (a) and antisense in (b) to (e).
(D) Quantitative RT-PCR analysis of SDG711 transcripts in inflorescence meristem (at different developmental stages), stem, leaf, and seedling. Levels relative to ACTIN transcripts are presented (y axis).