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. 2015 Jun 5;5:10903. doi: 10.1038/srep10903

Figure 7. Treatment of Gaucher fibroblasts with CoQ and PC NAdBT-AIJ increases GCase activity.

Figure 7

(a) GCase activities in control and Gaucher-B fibroblasts cultured in the absence or presence of CoQ (25 μM), PC NAdBT-AIJ (25 μM) and CoQ + PC (25 μM + 25 μM) for 96 h. GCase activities increase after CoQ, PC and markedly after CoQ+PC supplementation. Data, expressed as Gcase activity (% of control), represent the mean ± SD of 3 separate experiments. (b) GCase expression levels determined in control and Gaucher-B fibroblasts by Western blotting. Control and Gaucher fibroblast cultures were grown in normal culture medium or in medium supplemented with CoQ, PC and CoQ + PC for 96 h. Fibroblast protein extracts (50 μg) were separated on a 12.5% SDS-polyacrylamide gel and immunostained with an antibody against GCase. α-Tubulin was used as a loading control. (c) Densitometric analysis of Western blottings. Data represent the mean ± SD of 3 separate experiments. *p < 0.01 between control and Gaucher fibroblasts. ap < 0.05 between the presence and the absence of CoQ. bp < 0.05 between the presence and the absence of PC. cp < 0.05 between the presence and the absence of CoQ + PC. #p < 0.05 between CoQ + PC and CoQ or PC treatment.