Update Ayurveda 2014 Abstracts: Oral Presentation

. 2015 May;6(Suppl 1):S5–S25.

Update Ayurveda 2014 Abstracts: Oral Presentation

PMCID: PMC4456681

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/01: Oral delivery of Bromelain using calcium alginate beads

Devang Sarvaiya, Ujwala Shinde, Pankaj Jain, Sadhana Sathaye¹

¹Department of Pharmaceutics, Bombay College of Pharmacy, Santacruz, Kalina, Mumbai, ²Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, India

E-mail: ddsarvaiya@gmail.com

Background: The present research work was aimed at preparing microspheres for oral delivery of bromelain, an anti-inflammatory enzyme from pineapple fruit (Ananas comosus). Objective: The objective was to improve the oral delivery of this proteolytic enzyme by circumventing its instability in acidic environment and modulating its release in intestine for its anti-inflammatory action. Materials and Methods: Sodium alginate, calcium chloride, bromelain. Microspheres were prepared by ionotropic gelatin technique dropping the sodium alginate solution to magnetically stirred calcium chloride solution. Results: Optimized batch of microspheres were prepared using 3% sodium alginate concentration, 0.05M calcium chloride and curing time of 20 min with highest entrapment efficiency of 69.98%± 0.087%. In vitro release studies showed very low amount of drug release in 0.1N HCl and sustained release in simulated intestinal fluid pH 6.8 with more than 90% drug release at the end of 2 hrs. The drug release profiles for the optimized batch was best fitted with Korsmeyer-Peppas as well as Higuchi model based on regression coefficients. Bromelain microspheres showed 62.90%±1.44% edema inhibition in rat paw edema model in comparison to marketed bromelain formulation which showed 69.35% ±1.44% inhibition at end of 2 hrs. Optimized batch of microspheres were found to be stable as per ICH guidelines of stability. Conclusion: Thus alginate based microsphere drug delivery system can be effectively developed for oral delivery of bromelain and can be considered as an alternative approach to conventional NSAIDs and side effects associated with it.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/02: Analytical study to explore the pharmacokinetic aspect of Basti (Rectal drug administration)

Rohini Purohit, B. S. Prasad, Sreelakshmi Chaganti, Vedantam Giridhar

Department of Panchakarma, KLEU'S Shri BMK Ayurveda Mahavidyalay, Belgaum, India

Email: dr.rohiniayu@gmail.com

Background: Niruha basti is one among the prime Panchakarma procedures considered as ardha chikitsa and also referred as purna chikitsa involves rectal route of drug administration of the medicated formulation in a quantity of 250-300 ml (approx.). This treatment is widely being practiced and has been proved efficacious in various disorders like hemiplegia, sciatica, cardiac disorders, avascular necrosis, diabetes, obesity, rheumatoid arthritis, etc., with a retention period of 2 min-10 min (approx.) on an average as observed practically. Objective: A step forward now in research towards understanding the pharmacokinetics of basti which is frequently debated as a local action, placebo, etc., was tried to explore with the help of technology which is explained to have an action throughout the body. Materials and Methods: Madhutailika basti was selected as it has a single Kashaya dravya (medicine for decoction) as Eranda (Ricinus communis Linn.) in large quantity along with other drugs like honey, rock salt, moorchita tila taila (processed sesamum oil) and Shatapushpa (Anethum sowa Linn.) as kalka dravya. This basti was administered to the male wistar rats. Results: The decoction of Eranda and Madhutailika Basti which were subjected to UV analysis showed similar peaks. The plasma samples of the rats both with and without administration of basti showed a significant variation along with the peaks similar to that of the decoction and basti formulation on subjecting to UV analysis. Conclusion: These evidences pave way towards proving the systemic absorption of Madhutailika basti in turn implying the mode of action of Niruha basti (rectal drug administration).

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/03: Diosgenin ameliorates diabetes related renal injury through suppression of oxidative stress and inflammation

Divya M. Kanchan, Gauresh Somani, Vaibhavi Peshattiwar, Sadhana Sathaye

Institute of Chemical Technology, Department of Pharmaceutical Sciences & Technology, Matunga (E), Mumbai, India

Email: dmk86in@gmail.com

Background: Diabetes mellitus is a multi factorial metabolic disorder associated with genesis of diabetes related vascular diseases. Oxidative stress is claimed to be major impulsive factor in the etiology of these diabetic complications. Diosgenin, a steroidal saponin, reported to possess strong anti-inflammatory and antioxidant activity which may prove beneficial in combating oxidative stress and inflammatory responses generated in diabetes. Objective: The current study was undertaken to evaluate the effect of diosgenin in early hyperglycemia induced diabetic complications in kidney. Methods and Materials: Hyperglycemia was induced by streptozotocin (STZ, 45 mg/kg, i.p.) in rats followed by treatment with diosgenin (5, 10 & 20 mg/kg, oral 28 days). Biochemical analysis of oxidative stress parameters such as superoxide dismutase, catalase, reduced glutathione and lipid peroxidation (LPO) were evaluated in kidney homogenates.

Biomarkers of kidney damage such as microalbuminuria, kidney hypertrophy index and serum creatinine along with histopathology of kidney was evaluated. Myeloperoxidase levels were determined in kidney to evaluate the level of inflammation. Results: The altered oxidative stress levels in STZ induced diabetic rats were significantly ameliorated as manifested by decreased LPO and increased endogenous antioxidant levels by treatment of diosgenin in a dose dependent manner. Blood glucose was significantly decreased at 20 mg/kg. Microalbuminuria was significantly prevented by diosgenin signifying its protective effect on kidney which was confirmed by histopathological studies. Decreased myeloperoxidase levels in diosgenin treatment groups revealed its anti-inflammatory activity. Conclusion: The study proves diosgenin as a promising candidate in ameliorating diabetes associated complication through its anti oxidant and anti-inflammatory activity.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/04: Evaluation of anticonvulsant activity of Pistacia integerrima in zebra fish and rodent models of epilepsy

Pankaj Jain, Rufi Tambe, Mruniya Nahire, Atul Bharadwaj, Sadhana Sathaye

Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, India E-mail: pdjain23@gmail.com

Background: Epilepsy is a one of the common CNS disorder which is manifested by recurrent unprovoked epileptic seizures. At neuronal level, seizure activity occurs when glutamatergic excitatory neurotransmitters overrides gamma-aminobutyric acid (GABA) mediated inhibition. Objective: The objective of the present study was to evaluate Pistacia integerrima extracts for its anticonvulsant activity in zebra fish and rodent model of epilepsy. Materials and Methods: Petroleum ether (PEPI- 50, 100, 200 mg/kg) and methanolic (MEPI- 50 100, 200 mg/kg) extracts were evaluated in Pentylenetetrazol (PTZ) induced zebra fish model. The activity of the active extract was further confirmed in PTZ induced convulsions in mice. Further these extracts were screened in maximal shock induced (MES) convulsions. Results: PEPI exhibited dose dependent anticonvulsant activity in PTZ induced seizure in zebra fish model which was further confirmed in PTZ and MES rodent models. MEPI was ineffective in both the models of epilepsy. Conclusion: PEPI extract demonstrated potential anticonvulsant activity in zebra fish and rodent models. This study provides the evidence that anticonvulsant activity of PEPI extract could be due to modulation of GABA_A receptor or by its ability to block sodium channels.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/05: Validation of RP-HPLC methods for estimation of Lupeol, Wedelolactone and β -Asarone from Symplocos racemosa, Eclipta Alba, Acorus calamus and their marketed Ayurvedic and herbal formulations

Sunita Shailajan, Sasikumar Menon¹, Gauri Swar, Dipti Singh

Herbal Research Laboratory, Ramnarain Ruia College, Matunga, Mumbai, ¹Therapeutic Drug Monitoring Laboratory, Sion, Mumbai, India

E-mail: dips26098@gmail.com

Background: Symplocos racemosa Roxb. (Symplocaceae, bark), Eclipta alba Hassk. (Asteraceae, whole plant) and Acorus calamus Linn. (Araceae, rhizomes) have been used traditionally in the management of a wide range of ailments and are important component of Ayurvedic system of medicine. Lupeol, wedelolactone and ß-asarone are the active phyto constituents of the above mentioned plants. Various Ayurvedic formulations like Lodharasava, Pusyanuga Churna, Mahabhringaraj Taila, Mahavat Vidhvansan Rasa, Medhya Rasayana, etc., containing these plants are commercially available. Objective: The main objective of this study is marker-based standardization of these natural resources and their commercially available Ayurvedic formulations using validated RP-HPLC technique. Material and Methods: Separation of biomarkers lupeol, wedelolactone and ß-asarone was achieved using RP-HPLC-PDA technique and the methods were validated as per ICH guidelines. As an application of method, estimation of markers was carried out from Ayurvedic formulations and samples from different environments. Results: A good linear relationship was obtained for the calibration plots of lupeol, wedelolactone and ß-asarone. Methods were found sensitive for estimation of lupeol, wedelolactone and ß-asarone (LOD and LOQ values were nearly 0.5 and 1.0 μg/ ml respectively). The validated method was found to be simple, accurate, rugged and reproducible. These methods can be recommended for quality assurance and marker-based standardization of various plants, their parts and formulations containing these markers. Conclusion: These methods can be extended into quantification of plant phyto chemicals, checking authenticity of herbal formulations and facilitating routine quality control analysis of commercial Ayurvedic formulations.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/06: Macaranga peltata - Potential Wound Healing Agent

Minakshi Nehete, Pratima Tatke

Department of Pharmaceutical Chemistry, CU Shah College of Pharmacy, SNDT Women's University, Santacruz, Mumbai, India

E-mail: minakshi1277@yahoo.co.in

Background: Macaranga peltata is commonly known as Chandwar. In tribal medicine, gum powder of Macaranga peltata bark is used to join fractured bones. In a preliminary study in our laboratory, it is shown to possess antioxidant and wound healing activities. There are no literature reports with respect to scientific evaluation on Macaranga peltata. Objective: Objective of present study was to investigate in detail the antioxidant, antimicrobial and wound healing activity of Macaranga peltata. Materials and Methods: The powdered bark of Macaranga peltata was extracted with various solvents using soxhlet apparatus. Prepared extracts were screened by determining total phenolic content (TPC) using Folin-Ciocalteu phenol reagent method. Methanol extract showing highest TPC was evaluated for detailed antioxidant and antimicrobial activity against wound pathogens. A topical gel was formulated by incorporating methanol extract in suitable gelling agent. Wound healing activity of this gel was studied by using excision and incision wound models. Betadine was used as a standard. Results: The methanolic extract showed potent antioxidant and antimicrobial activity.

The statistical analysis indicated that wounds treated with gel containing 1% w/w methanolic extract exhibited significant increase in tensile strength (P < 0.001) as compared to control. The epithelialization period was found to be less as compared to control. The results demonstrated that Macaranga peltata displayed promising wound healing activity. Conclusion: A topical gel formulation containing methanol extract of bark of Macaranga peltata was prepared and evaluated. The gel exhibited excellent wound healing activity on fresh wound in rats.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/07: Cytochrome P450 inhibition potential of Trigonella (Fenugreek), Trachyspermum (Ajwaine) and Nigella Sativa (Kalonji) in rat liver microsomes

Dipali Mendhe, Madhav Singh Seervi, Sadhana Sathaye

Pharmacology lab II, Institute of Chemical Technology, Matunga, Mumbai, India

E-mail: dips.mendhe@gmail.com

Background: The use of natural products as herbal medicines has increased over the last decade. Such widespread use of herbal medicinal products throughout the world has raised serious questions concerning the quality, safety and efficacy of these products. Literature survey suggested the crucial role of Cytochrome P450 in drug metabolism and drug-herb interactions as approximately 80% of the drugs are metabolized by the same. Trigonella foenum (Fenugreek), Trachyspermum copticum (Ajwain) and Nigella sativa (Kalonji) are very commonly used Indian spices and widely used in traditional herbal medicines as dietary supplements. Objective: The present study was undertaken to investigate the effects of methanolic extracts of the above mentioned herbs on CYP1A2, CYP2D6 and CYP3A4 in rat liver microsomes in vitro. Materials and Methods: Rat liver microsomes were prepared by calcium aggregation method. Microsomes were then characterized for the ethoxy resorufin-O-deethylase, dextromethorphan demethylase and testosterone hydroxylase activities. Inhibition potential of Trigonella foenum, Trachyspermum copticum and Nigella sativa extract were evaluated. Results: The result suggested that Trigonella, Trachyspermum and Nigella extract did not show any inhibition towards CYP1A2, CYP2D6 and CYP3A4. The IC₅₀ Values for Trigonella, Trachyspermum and Nigella were found to be 515, 188 and 136 µg/ml for CYP1A2, 827,107 and 47.4 µg/ml for CYP2D6 and >500, >500 and >500 for CYP3A4 respectively. Conclusion: It can be concluded that the above mentioned herbs are safe to use along with other drugs as they exhibited no significant inhibition towards CYP1A2, CYP2D6 and CYP3A4.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/08: PCOS-Merging Ayurvedic and Allopathic Management

Jayshree S. Dawane, Vijaya A. Pandit, Kapil S.Khade, Sonali P. Suryawanshi, Asmita Wele¹, Madhuri Dalvi¹, Supriya Bhalerao²

Department of Pharmacology, BVDU Medical College, Pune, ¹Department of Rasashastra and Bhaishajyakalpanavigyan, BVU College of Ayurveda, Pune, ²IRSHA, BVU Pune, India

E-mail: jayshreedawane@gmail.com

Background: Polycystic ovarian syndrome (PCOS) is a complex endocrine disorder characterized by elevated levels of androgens, insulin resistance, anovulation, amenorrhoea or oligomenorrhoea, obesity and Polycystic ovaries. Objective: To evaluate efficacy of Ayurvedic formulations Nishamalaki and Gugul in PCOS induced in wistar rats. Material and Method: Nishamalaki, was prepared from powder of Curcuma longa and fresh juice Emblica officinalis according to Ayurvedic literature and administered with honey. PCOS was induced in female rat's with1mg/kg body weight letrozole for 28 days. Vaginal smears collected daily from all the groups and studied for changes in estrous cycle. In one group i.e. Group I (Control), rats were treated only with Saline, Letrozole treated 30 female rats were divided into 5 groups Group II was (Control) PCOS Induced (No treatment), Group III was Clomiphene citrate, Group IV was Nishamalaki, Group V was Nishamalaki and Metfromin and Group VI was Nishamalaki, Metfromin and Gugul and treatment was given orally according to groups for 42 days. Body weight, food intake, insulin, blood glucose levels was checked before and after treatment. Results: PCOS induced rat showed increase in body weight and insulin levels. Lipid profile was altered in these animals. Nishamalaki reduced serum cholesterol (P < 0.05), triglycerides (P < 0.01) and LDL levels (P < 0.01). The body weight loss (P < 0.001) was more significant with Nishamalaki treatment. These findings demonstrate that levels of serum glucose, cholesterol and triglycerides decreased significantly in PCOS treated rats with Nishamalaki as compared to Clomiphene citrate. Conclusion: Nishamalaki is the most promising drug in PCOS, it has reduced weight, normalised lipid profile and oestrus cycles.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/09: Protective action of Carissa Carandas in Gentamicin induced nephropathy via attenuation of oxidative stress

Jayesh B. Dhodi, Deepavali R. Thanekar, Snehal N. Mestry, Archana R. Juvekar.

Institute of Chemical Technology, Department of Pharmaceutical Sciences and Technology, Matunga, Mumbai, India

E-mail: jayeshdhodi2@gmail.com

Background: Oxidative stress is reported to play a pivotal role in the pathogenesis of nephropathy, one of the co-morbidities associated with diabetes. A previous study carried out on methanolic extract of fruits of Carissa carandas (MCCF) demonstrated the ameliorative effects in diabetes induced nephropathy in rats. Thus, in order to understand the mechanism of action of MCCF, it was evaluated in gentamicin (GM) induced nephropathy in rats. Gentamicin induces acute kidney injury through oxidative damage produced by free radical generation. Objective: To evaluate the effect of MCCF on oxidative stress in gentamicin induced nephropathy. Materials and Methods: Male Sprague Dawley rats were divided into five groups; Group I - normal control (received distilled water, p.o.), Group II - negative control (gentamicin 80 mg/kg i.m., 8 days), group III, IV and V were administered 100, 200 and 400 mg/kg MCCF p.o. respectively along with gentamicin 80 mg/kg i.m. for 8 days respectively. Biomarkers of nephropathy were assessed in serum and urine. Oxidative stress parameters, superoxide dismutase, catalase, reduced glutathione and lipid peroxidation were evaluated in kidney homogenates. The extent of kidney damage was analyzed by histo-pathological study. Results: MCCF significantly improved the altered oxidative stress levels in gentamicin induced nephropathy in a dose dependent manner as compared to negative control. The progression of nephropathy was markedly inhibited as demonstrated by decreased levels of biomarkers in serum and urine and the histo-pathological analysis. Conclusion: MCCF ameliorated the development of nephropathy by suppressing oxidative stress in gentamicin induced nephropathy in rats.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/10: Saraca Indica fraction prevents cataract development and progression in Streptozotocin-induced diabetic rats: an aldose reductase inhibitory activity

Sadhana Sathaye, Gauresh Somani

Pharmacology Research Laboratoy-II, Department of Pharmaceutical Sciences and Tech, Institute of Chemical Technology Mumbai, India

E-mail: sadhanasathaye@hotmail.com

Background: Cataract, characterized by cloudiness or opacification of the eye lens is the leading cause of blindness in developed and developing countries. Diabetes is one of the prime causes for cataract development. Objective: The present study was designed to investigate the effect of bioactive fraction of Saraca Indica flowers (SIF) extract on development and progression in streptozotocin (STZ) induced diabetic rats. Materials and Methods: The inhibitory effect of SIF extract and its fraction was tested for inhibition against rat lens aldose reductase (AR). Furthermore, active fraction was investigated against high glucose induced opacification of lens in lens culture and STZ induced diabetic cataract in rats. Identification of the bioactive components was attempted using different analytical techniques such as HPTLC, HPLC, LC-MS and GCMS. Docking pattern and binding interactions of the bioactive components gave valuable insights on their mechanism of action. Results: Ethyl acetate fraction produced maximum inhibition of aldose reductase activity. Treatment with SIF fraction at a concentration of 1 mg/ml lowered lens opacity in 72 hrs. In vivo experiments were performed with STZ induced diabetic rats. Prolonged treatment with ethyl acetate extract of SIF to STZ-induced diabetic rats decreased the blood glucose levels and inhibited the AR activity and delayed cataract progression in dose dependent manner. Conclusion: The present study shows that ethyl acetate extract of SIF has pharmacologically active components with a potential to inhibit rat lens AR activity.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/11: Assessment of antimicrobial properties of hydro alcoholic extract of Morinda citrifolia

Rajesh Kumar Suman, Ipseeta Ray Mohanty, Vijay Mane, Y. A. Deshmukh, A. D. Urhekar

MGM Medical College, Kamothe, Navi Mumbai, India

E-mail: rajeshsuman2043@gmail.com

Background: Morinda citrifolia is a versatile medicinal plant with a broad spectrum of pharmacological activities and traditionally being used for the treatment of various diseases. Increasing emergence of resistance to the currently available antibiotic has necessitated continuing search for new antimicrobial compound. Hence, the present work was designed to investigate the antimicrobial activity of extract of Morinda citrifolia. Objective: To evaluate antimicrobial activity of Hydro alcoholic fruit extract of Morinda citrifolia against E. coli, Psedomonas aeroginosa and Acinatobactor. Material and Methods: The Hydro alcoholic extract of Morinda citrifolia (Fruit) was purchased from Sanat Pharmaceutical, New Delhi. The extract was tested against standard strains and clinical isolate of E. coli, Pseudomonas aeroginosa and Acinatobactor by Agar well diffusion technique using Muller Hinton agar. Different concentration of test drug 1mg, 2 mg, 4 mg and 8 mg were tested. Cefotaxime was used as control drug. Results: The Hydro alcoholic extract of Morinda citrifolia at concentration of 1 mg, 2 mg, 4 mg and 8 mg were tested against three bacteria. E. coli (ATCC 25922) and E. coli from clinical isolate (Urine), were significant sensitive with test drug as compared with control drug. The Pseudomonas aeroginosa (ATCC 27853) and Pseudomonas aeroginosa of clinical isolate (Pus) were also significant sensitive by Test drug. Against, Acinatobactor from clinical isolate (Tracheal Secretion), the test drug did not demonstrate antimicrobial activity at 1 mg, while it showed significant activity at Conc. of 2 mg, 4 mg and 8 mg as compared with control drug. Conclusion: The herb Morinda citrifolia was found to have significant antimicrobial properties against test bacteria.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/12: Anti-epileptogenic and anti oxidant effect of Borneol: assessment in pentylenetetrazole induced kindling model of epilepsy

Rufi Tambe, Pankaj Jain, Sadhana Sathaye

Pharmacology Lab-II, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, India Email: rufitambe@gmail.com

Background: Borneol, a bicyclic monoterpene has been found to exhibit a positive GABA modulatory activity at human recombinant GABA receptors. Borneol showed neuroprotection on cortical neurons against oxygen-glucose deprivation/reperfusion in vitro and exhibited anti-inflammatory and anti-oxidant activity on cortical neurons. Objective: The present study was aimed at investigating the anti-epileptogenic effect of Borneol in the pentylenetetrazole (PTZ) induced kindling model along with its ability to suppress oxidative stress and neuro-inflammatory marker glial fibrillary acidic protein (GFAP). Materials and Methods: Borneol (5, 10, 25 mg/kg, i.p.) was evaluated on the course of kindling followed by estimation of oxidative stress markers like superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT) and lipid peroxidation (MDA) in PTZ kindled mice. GFAP was also evaluated at the end of the study using immunohistochemistry. Results: The results showed that treatment with Borneol significantly decreased the PTZ induced seizure score. The biochemical alterations induced by PTZ were ameliorated in Borneol treated animals which was indicated by decreased malondialdehyde levels and increased glutathione, catalase and superoxide dismutase levels. It also alleviated the levels of GFAP as manifested by less immunostaining for the same. Conclusion: The above results are suggestive of the anti-epileptogenic potential of Borneol and it being a promising candidate in the treatment of epilepsy.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/13: Punica Granatum Fruit peel: A potential candidate for holistic treatment in inflammatory disorder of Intestine

Deepali Mahajan, Usha Satyapal, Pratima Tatke, Vikram Naharvar

Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, S.N.D.T Women's University, Santacruz, Mumbai, India

E-mail: deepali.mahajan@gmail.com

Background: Punica granatum fruit, commonly called pomegranate belongs to family Lythracea. In Ayurveda its peels are used in treatment of diarrhea and dysentery. The peels are rich in polyphenols such as tannins and flavonoids. Objective: The objective of the present is to study the effects of methanolic extract of pomegranate peels (ME-PP) in diarrhea, intestinal inflammation and pain. Materials and Methods: The anti diarrheal effect of ME-PP was investigated by measuring inhibitory action on diarrhea and intestinal fluid accumulation induced by castor oil; and intestinal motility in rats. Effectiveness against intestinal inflammation induced by intra rectal instillation of TNBS was assessed in mice. The extent of colonic injury was evaluated by colonic macroscopic damage scores, weight/ length ratio, histopathology; diarrhea score, feed intake and bodyweight of mice. The colonic inflammatory response was assessed by TNFa and IL1 -β; and oxidative stress by malondialdehyde (MDA) levels. The analgesic effects were evaluated by writhing test in mice. Results: ME-PP showed dose dependent effect against diarrhea, intestinal inflammation and pain. Administration of ME-PP delayed the onset of diarrhea, reduced the intestinal motility, and decreased the intestinal fluid accumulation. The colonic mucosal injury and inflammation was significantly reduced by ME-PP as evidenced by reduced colonic damage, weight/ length, cytokine levels (TNF-α and IL1-β) and MDA levels. Analgesic effect of ME-PP was demonstrated by reduction in the writhing which can be correlated to inflammatory pain associated with intestinal inflammation. Conclusions: ME-PP can be a potential holistic treatment for treating inflammatory disorders of intestine and relieving the associated symptoms such as diarrhea and pain.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/14: Marker-based standardization of two ayurvedic formulations: Arka vati and Lavangadi vati

Sunita Shailajan, Sasikumar Menon, Mayuresh Joshi, Bhavesh Tiwari

Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai

E-mail: m2j5288@gmail.com

Background: Arka Vati (AV) and Lavangadi Vati (LV) are traditional Ayurvedic formulations used in the treatment of indigestion, digestive impairments caused by Kapha Dosha, allergic respiratory conditions, etc. Though the formula composition and therapeutic claims of these formulations are part of Ayurvedic Formulary of India, validated scientific methods for its quality and safety evaluation are yet to be documented. Objective: Present study was aimed to carry out marker-based standardization of AV and LV using validated HPTLC method. Material and Methods: Standardized operating procedure for the preparation of AV and LV were developed in accordance with Ayurvedic Formulary of India. Physicochemical and preliminary phyto chemical evaluation was carried out as per reported methods. Chromatographic evaluation of these formulations was performed using validated HPTLC method in terms of their piperine content (piperine a major component of common ingredient Marica). As an application of this method, in-house formulations were compared with the formulations available in the market. Safety of the formulation was evaluated in mice as per OECD guideline no. 420. Results: SOPs’ of both the formulations has been established. Content of piperine was determined from both the formulations using a simple, rapid and accurate HPTLC method. Significant variation in the piperine content was observed in various AV and LV formulations. Conclusion: The results of quality control parameters obtained after scientific evaluation of AV and LV can be used as reference standard for quality control/assurance in laboratories of pharmaceutical firms in order to have a proper quality check over their preparation and processing.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/15: Hridayavarga Aushadhi-Carissa carandas: Evaluation of its antihypertensive activity

Kalyani H. Barve, Tejas Rami

Shobhaben Pratapbhai Patel- School of Pharmacy and Technology Management, SVKM's NMIMS, Mumbai, India

E-mail: kalyani.barve@nmims.edu

Background: Carissa congesta belonging to Apocynaceae, is known as karamarda (Sanskrit). It is found throughout India. The root is credited with stomachic, anti diarrheal, anthelmintic properties and contains salicylic acid, lignin and sesquiterpenes: carissone and carindone. However, in Ayurvedic and Unani system of medicine it is reported to have an effect on the cardiovascular system, but no scientific evidence exists for the same. Even the Charaka samhita mentions this as Hridayavarga class Objective: To evaluate Phyto-chemical and pharmacological comparison of root and bark extract and Co-relation of therapeutic activity with phyto-constituent of bark and root. Materials and Methods: Alcoholic extract of bark (BE) and root (RE) was prepared. The extracts were screened for detection of different phyto-constituents and subjected to fingerprinting by TLC. The proposed active phyto-constituents of the extracts were evaluated by molinspiration software to predict the bioactivity and were then evaluated at 250mg/kg, to check the antihypertensive effect in cadmium chloride induced hypertensive animals. Results: The extracts showed the presence of steroids, glycosides, tannins and alkaloids. The BE showed a blood pressure of 94.97 + 11.94 mm Hg whereas the RE showed a blood pressure 67.33 + 8.79 mm Hg against 115.88 + 4.88 mm Hg pressure in hypertensive rats. Carrisone was the major phyto-constituent as revealed by the fingerprinting of BE and RE.It further showed nuclear receptor binding activity and enzyme inhibitory activity. Conclusion: The study proves that the BE and RE of Carissa carandas possesses antihypertensive activity. However, further investigation are being undertaken to find the exact mechanism.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/16: An upshayatmaka trial of Shreephaladi Vati and Marichyadi Churna in Grahani dosha

Anita Meghwal, Nadira Khatoon, Pawankumar Godatwar’ Sevatkar Balkrishan, Sisir Kumar Mandal

Department of Roga and Vikriti Vijnana, National Institute of Ayurveda, Amer Road, Jaipur, Rajasthan, India

E-mail: anitameghwal09@gmail.com

Background: In the recent years, people are living with sedentary lifestyle. Hence list of lifestyle generated disorders are increasing day by day. Grahani dosha that is a psychosomatic disorder caused by regular intake of junk food that weakens the digestive fire and disharmony is produced between the digestion and absorption of body. Objective: To conduct an upshayatmaka trial of Ayurvedic drugs on grahani patients. Materials and Methods: Total 60 randomly selected cases, excluding dropouts were taken and assigned into two groups (Group A and B). In Group A Shreephaladi Vati was given in a dose of 2 Vati twice daily after food with Takra as anupana and in Group B Marichyadi Churna was given in a dose of 3 g twice daily after food with Takra as anupana. Total duration of treatment was 2 months with the regular follow-up of an interval of 15 days for observing any side effect of drugs. Results: Shreephaladi Vati on subjective parameters showed statistically extremely significant (P < 0.0001) improvement in Drava mala, muhur muh ur mala, Abdominal pain/discomfort, mucous in stool, gas/flatulence, odor in stool, arochaka and balakshaya, while Marichyadi Churna showed statistically less significant improvement in mucous in stool, gas/flatulence and balakshaya Conclusion: Both Shreephaladi Vati and Marichyadi Churna significantly reduced the symptoms of grahani dosha, although Shreephaladi Vati was much more effective in reducing the subjective variables of Grahani Dosha.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/17: Studies on Pravala Moola and Shakha Bhasma and Pisti w.s.r. to pharmaco-kinetics

Puja Sansare, K. Shankar Rao

Department of Rasashastra, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: pujasansare@rediffmail.com

Background: Pharmacokinetics is a branch of pharmacology that deals with the factors that establishes a relationship between drug dosage and variation of drug concentration at the site of action. Pravala is a well-known drug in Ayurvedic literature since ages, and it has been used in various forms such as Bhasma and Pisti. For the present study, Pravala Bhasma and Pisti of both Shakha and Moola have been evaluated for its absorption and efficacy through the pharmacokinetics. Objective: To assess the absorption pattern of all the Pravala samples in serum at certain intervals. Materials and Methods: The pharmacokinetics study was conducted on dogs so as to determine the different plasma concentration of calcium after oral administration of test drugs. The amount of calcium present in the serum before and after administration of samples was assessed. Further assessment was done at an interval of ½, 1^st, 2^nd, 3^rd, 4^th, 5^th, 6^th, 7^th and 8^th h. Results: The results showed that initially serum calcium level started increasing after ½ h of drug administration. The peak level of serum calcium reached at 3^rd h and it went down subsequently till 6^th h. At 7^th and 8^th h, the serum calcium level reached baseline. Conclusion: Overall the present study suggests that the Pravala Moola bhasma showed comparatively better results in increasing serum calcium levels.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/18: Clinical evaluation of the role of tablet cardicare and Basti treatment in the management of coronary artery disease (Hridyaroga)

Hemant Narwade, R. K. Joshi

Department of Kayachikitsa, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: hemantsnarwade@gmail.com

Background: In India, approximately 70 million people suffer from coronary artery disease (CAD). CAD can be correlated with Hridyaroga, which has been described in Ayurvedic texts. WHO considered it as a modern epidemic as it is fourth leading cause of death. As allopathic treatment of CAD need group of medicines having a lot of side effects, so there is a need of some herbal formulation. Objective: Present study was done to assess the efficacy of herbal formulation tablet cardicare and Basti treatment in comparison to standard drug diltiazem (tablet dilzem) on the basis of subjective and objective criteria. Materials and Methods: Totally, 30 registered patients were divided into three groups (each containing 10 patients) Group 1 patients received tablet dilzem (diltiazem hydrochloride) 30 mg TDS for 30 days. Group 2 patients received tablet cardicare 2 tablets TDS with lukewarm water for 30 days and Group 3 patients received tablet Cardicare for 30 days and Basti (kalakrama) with Dashmula taila and Dashmula kwatha. Patients were followed up after 15 and 30 days of treatment. Result: Group 2 and Group 3 patients showed highly significant result and Group 1 patient showed significant result in symptoms of CAD “grading scale” provided by “Canadian cardiovascular society” with changes in lipid profile, electrocardiogram, and computerized treadmill test. Conclusion: The trial drug tablet. Cardicare having cardio-protective and anti-angina action along with Basti treatment showed significant results in the management of CAD (Hridroga) as seen with its effect on various scientific parameters.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/19: Pharmacological evaluation of antipyretic and analgesic activity of Pippali (Piper longum linn.) fruit lavigated with cow's urine

Rabinarayan Acharya, Kapil Lathiya, Rasika Kolhe, Mukesh Nariya

Department of Dravyaguna, IPGT and RA, GAU, Jamnagar, Gujarat, India

E-mail: drrnacharya@gmail.com

Background: In Ayurveda, Pippali (Piper longum L) and Gomutra have significant therapeutic values in many diseases. Bhavana (lavigation) is a procedure intended to increase the efficacy of the drug or to get the desired effect. In folklore practice, Gomutra Bhavita Pippali (Pippali lavigated with cow's urine) is used in the management of fever. The growing side effects of the synthetic drugs necessitate the research for the evaluation of safe, effective and fast-acting analgesic and antipyretic herbal remedies. Objective: To evaluate the antipyretic and analgesic effect of Pippali fruits lavigated seven times with cow's urine in the experimental model. Materials and Methods: For each study, Charles Foster rats of both the sex were divided into three groups of six animals each. For anti-pyretic and analgesic effect; Brewer's yeast induced pyrexia, and Tail flick method was adopted respectively. The test drug was administered orally at a dose of 540 mg/kg and the activities were compared with standard control in both models. Result: In antipyretic study, 1 h after Brewer's yeast administration in rats, standard control paracetamol group produced significant decrease (110%) and Gomutra Bhavita Pippali produced (96%) decrease in rectal temperature but insignificant (48.9%) effect was seen in Gomutra Bhavita Pippali after 3 h in comparison to control group. Gomutrabhavita Pippali produced non-significant increase in tail flick response in comparison to control group, as well as initial values in evaluation of its analgesic effect. Conclusion: Gomutra Bhavita Pippal produced significant antipyretic and moderate analgesic activity.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/20: A preliminary phytochemical evaluation of leaf and seed of Ipoemia pentaphylla linn

Minutee Patel, Chetan Kamble, Sudipta Roy, Anagha Ranade, V. J. Shukla, R.N. Acharya

Department of Dravyguna, IPGT and RA, GAU, Jamnagar, Gujarat, India

E-mail: ckkamble89@gmail.com

Background: The authentic identification and standardization of Ayurvedic drugs is a very essential part to assess the genuinity of the crude drugs. Folklore claims, an integral part of traditional systems of medicine, are needed to be validated thoroughly. The phytochemical analysis of any drug is a primary step toward its authentication. Different parts of Ipoemia pentaphylla Linn., a folklore medicinal plant, not reported for its chemical nature, is reported for its blood purifier, anti-inflammatory actions. Objective: To assess the analytical parameters of I. pentaphylla leaf and seed. Materials and Methods: The study included primary organoleptic character evaluation followed by physicochemical analysis constituting determination of loss on drying, total ash, alcohol soluble extractive value, pH, etc. Qualitative tests were done to identify the presence of various chemical constituents followed by quantitative estimation of protein and HPTLC study. All the studies were carried out following standard laboratory parameters. Results: The study revealed the presence of higher moisture content in the seeds than in the leaf Ash values of seed and leaf were found to be 26.31%w/w and 16.43% w/w at 450⁰C. Water-soluble extractive value of leaf was higher while alcohol soluble extractive value was higher in seeds. Preliminary qualitative tests revealed presence of alkaloids, tannin, steroids, flavonoids, carbohydrates, etc., in leaf and amino acids and protein in seed. Quantitative protein estimation and HPTLC study were also performed. Conclusion: The present observations may be considered as a standard parameter for preliminary phytochemical values of I. pentaphylla Linn.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/21: Krimi chiktsa in Amlapitta and its significance - a statistical analysis

Neha Sharma, Sameer Naik

Department of Kayachikitsa, KLE University's Shri BMK Ayurveda Mahavidyalaya, Shahapur, Belagaum, Karnataka, India

E-mail: dr.nehasharma24@gmail.com

Background: In Ayurveda, Amlapitta is a disorder considered for gastric irritation, abdominal pain, etc. Amlapitta is a conditional disorder that is considered as precursor of Grahani roga and for grahani roga, Krimi chiktsa is mentioned. Objective: To analyze whether the Krimi chikitsa can show a significant result in Amlapitta or not. Materials and Methods A prospective study was carried out in individuals having chief complaint of Amlapitta. Totally, 105 subjects were randomly selected strictly on the basis of inclusion and exclusion criteria who visited the OPD in the year 2013 and 2014 at our institute. Among them, 32 belonged to vata predominant prakriti (30.47%), 53 pitta predominant prakriti (50.47%), 20 kapha predominant prakriti (19.04%). All patients were treated with Krimikuthara rasa and Vidangarista. Results: It was observed that the chief symptoms of Amlapitta that is, burning sensation, abdominal pain, arochaka were reduced up to 30–40%. Conclusion: The study reveals the beneficiary role of Krimi Chikitsa in Amlapitta patients.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/22: Wound healing activity of standardized methanolic extract of Randia dometorum (rubiacae)

Chhaya Gadgoli, Priyanka Gosavi

Department of Pharmacognosy, Saraswathi Vidya Bhavan's College of Pharmacy, Dombivli, Mumbai, Maharashtra, India

E-mail: chhayahg@rediffmail.com

Background: Randia dometorum fruits are commonly called as Madana, utilized in folklore medicine for treatment of ulcers, inflammation, wounds and skin diseases. Objective: The present study was carried out with the aim to evaluate the wound healing activity of methanolic extract of fruits of R. dometorum. Materials and Methods: The fruits were extracted successively with methanol and water. The methanolic extract was incorporated in simple ointment base in the concentration of 1 and 2% w/w and evaluated for the wound healing activity in rats using excision and incision wound models. The monitoring of wound healing process was done through determination of parameters viz., period of epithelization and hydroxyproline content in eschar in excision wound model, while in case of incision wound model the tensile strength of newly formed skin was determined. A triterpenoldal aglycon RD1 isolated from the metahnolic extract was utilized for the standardization of the extract. Results: The methanolic extract was found to contain 0.45% w/w of RD1 that was characterized to be oleanolic acid. The methanolic extract in the concentration of 2% w/w exhibited good wound healing activity as significant (P < 0.001) reduction in the epithelization period and significant (P < 0.001) increase in the hydroxyproline content and the tensile strength, was observed as compared to the control group treated with the simple ointment base. Conclusion: Methanolic extract of the fruits of R. dometorum has good potential as wound healing agent and is rich in saponins.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/23: Gastro protective effect and biomarker-based pharmacokinetic profile of an Ayurvedic formulation: Bilvadileha

Sunita Shailajan, Archana Matani

Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai, Maharashtra, India

E-mail: sampatarchana@yahoo.com

Background: The high risk of developing gastric ulcers by non-steroidal anti-inflammatory drugs has affected their common application in the treatment of various inflammatory disorders. Several indigenous plants and formulations have been described in Indian systems of medicine for such symptoms. Bilvadileha is an Ayurvedic semi solid formulation prescribed for the treatment of digestive impairment, tastelessness, emesis and excessive salivation. Objective: Though composition and therapeutic claims of Bilvadileha are part of the Ayurvedic Formulary of India, the scientific validation of its pharmacological profile is yet to be documented. Materials and Methods: Chromatographic standardization was carried out using HPTLC, which included the determination of piperine content in Bilvadileha along with the evaluation of its stability samples for different storage periods. Acute toxicity studies were conducted in mice as per OECD guidelines No. 420. Evaluation of the preventive effect of Bilvadileha on indomethacin induced (30 mg/kg p.o.) experimental gastric ulcer in rats (180–200 g) was carried out and compared with that of synthetic anti-ulcer drug Omeprazole (10 mg/kg). Pharmacokinetics of piperine from Bilvadileha after oral administration to rabbits was evaluated using validated high-performance liquid chromatography method. Results: Piperine content in Bilvadileha was found to be 0.72 ± 0.02 mg/g using HPTLC. Safety of the formulation was affirmed in mice at 2 g/kg. Aqueous slurry of Bilvadileha prevented ulcer formation in indomethacin-induced ulcer model. Piperine was detected in rabbit plasma with C_max and T of 79.21 ± 1.24 ng/ml and 0.75 h respectively post administration of Bilvadileha. Conclusion: The current work demonstrates modern approach toward standardization of traditional Ayurvedic formulation Bilvadileha.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/24: Correlation between Mamsa sarata and muscle strength

Jitendra Rajput, R. R. Deshpande

Department of Kriya Shareer, College of Ayurved and Research Centre, Nigdi, Pune, Maharashtra, India

E-mail: dr.jitendra8rajput@gmail.com

Background: In the Dashvidha pariksha of Ayurveda, it has stated that Saar pariksha is one of the important investigation that is, examination for the strength. To understand the quantitative and qualitative strength of body, Ayurveda has specifically explained the Dhatu Sarata concept. Dhatu quality is important for immunity aspect and body strength. Ayurveda says Mamsa Sara person has a good quality Bala. Bala can be translated in many ways as muscle strength or stamina or muscle flexibility. Strength of the body can be explained as “Bala”. So, the topic has been selected to find out the relation of Mamsa Sarata and “Muscle strength” by grip dynamometer. The practical applicability of this study will be the availability of objective parameter for Bala, mentioned in Mamsa Sarata examination. Materials and Methods: Totally, 50 healthy volunteers were selected. Mamsa Sarata was taken out with the help of the conventional method (questionnaire). Grip dynamometer was used for muscle strength measurement. Both the readings were co-related with each other. Results: While doing the study, it was noticed that muscle strength could be co-related with mamsa sarata. Uttam mamsa sara people have good muscle strength while heen mamsa sarata person have less muscle strength. Conclusion: So grip dynamometer can be used instead of mamsa sarata questionnaire for taking out mamsa sarata and muscular status of a person. This could be very useful and simple and time-saving process. This will help in updating Ayurveda with the modern technology also.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/25: Role of modern analytical validation of Ayurveda Sidhipariksha for ayurvedic formulation w.s.r. to Bhasma form

Atul Dnyandeo Sanap, J. M. Takalikar

Department of Rasashastra and Bhaishjya Kalpana, Loknete Rajarambapu Patil Ayurvedic Medical College, Hospital, Post Graduate Training and Research Center, Urun-Islampur, Sangli, Maharashtra, India

E-mail: samap067@gmail.com

Background: As requirement of increased Ayurvedic drug use, Ayurvedic pharmaceutical company has to establish more production of Ayurvedic formulations (Drugs) within the short period. These productions have affected the properties and action of Ayurvedic medicine. Also, as the requirement increases the genuine raw material is hard to get. In such cases, substandard products may be manufactured specially the Bhasmas. To hurry in supply the same they show low or null medicinal effect. This produced bad image of Ayurveda at national and international level. For avoiding these, there is need to establish standards for it. Objective: To study Ayurvedic Bhasma Pariksha in Ayurvedic view, to study the modern analytical parameters for Ayurvedic Bhasma, to correlate the Bhasma Pariksha and analytical parameters for Ayurvedic Bhasma. Materials and Methods: The modern analytical parameter like XRD, XRF, SEM-EDX, AAS were used and correlated with Ayurvedic Bhasma Pariksha such as Varitaratva, Apunarbhavatva, Rekhapurnatva, Mrutaloha, Nirutha, Uttam Pariksha, Amla Pariksha. E.g. Vanga Bhasma is in oxide form (SnO) with about 98% which can be correlated with apunrbhavatva and practical size correlates with Rekhapurnatva. Result: These show that the modern analytical parameters tests are significant for Ayurvedic Bhasma or vice-a-versa. Conclusion: There is a need to prove and establish the standards of Ayurvedic Bhasma with the help of modern parameters.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/26: A randomized control trial of Arjuna and Agnimantha on metabolic syndrome

Sachin Kumar, Pawankumar Godatwar, Himmat Lal Dhaker

Department of Rognidana, National Institute of Ayurveda, Jaipur, Maharashtra, India.

E-mail: drsachinrai1983@gmail.com

Background: Metabolic Syndrome has received increased attention more and more in the past few years globally and is the burning problem of today's world. It is a set of risk factors that includes abdominal obesity, insulin resistance, hypertension and dyslipidemia. Objective: To evaluate the efficacy of Arjuna churna and Agnimantha churna in metabolic syndrome. Methods: Totally, 120 patients were selected randomly and divided into two groups. Group A patients were treated with Arjuna churna. Group B patients were treated with Agnimantha churna. Chief symptoms of metabolic syndrome along with prakriti and bala were taken into consideration. The patients were diagnosed for metabolic syndrome on the basis of criteria laid down by International Diabetes Federation (2005). Results: In Group B, chief symptoms of metabolic syndrome that is, Chala sphika udara stana, Krichha vyavaya, Kshuda vriddhi, Nidradhikya, Kshudra swasa, Alpa bala, Alasya, Bahu mutrata, Hriddrava, Kosthabaddhata showed highly significant improvement, while in Group A only Kshuda vriddhi Svedadhikya and Kosthabaddhata showed significant improvement. Biophysical parameters such as body weight, body mass index, visceral fat, body fat, Basal metabolic rate, Waist hip ratio, pulse rate, respiratory rate and blood pressure (systolic and diastolic) were assessed and significant results were obtained in both the groups. Conclusions: Arjuna churna showed better results in Bhrama, Shirah shoola, Hriddrava, while Agnimantha churna showed better results in most of the other major signs and symptoms of Metabolic syndrome.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/27: Rosa damascena flower petals: A potential agent for oxidative stress related disorders

Priti Patil, Pratima Tatke, S. Y. Gabhe¹

Department of Pharmaceutical Chemistry, C. U. Shah College of Pharmacy, S.N.D.T Women's University, Santacruz, Mumbai, ¹Department of Pharmaceutical Chemistry, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra, India

E-mail: patilpriti25@gmail.com

Background: Rosa damascena mill (Rosaceae) is known for its perfumery and cosmetic applications. Medicinal properties of anti-HIV, antibacterial and antioxidant activities of R. damascena have been demonstrated. Rose petals contain volatile oils, glycosides, phenolic such as flavonoids that are responsible for its free radical scavenging activity. Objective: The main objective of the study was to evaluate the free radical scavenging activity of R. damascena flower petals by various in-vitro free radical scavenging assay methods. Materials and Methods: Ethanol, chloroform, acetone and aqueous extracts of fresh and dried Rose petals were prepared and evaluated for the presence of total phenolics in terms of Gallic Acid Equivalent. Ethanol and acetone extract with high phenolic content were selected for various in vitro free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6-sulfonate] (ABTS), pyrogallol red bleach by peroxynitrite inhibition, xanthine oxidase inhibition (XOI), superoxide scavenging and lipid peroxidation assay. Results: All the extracts of fresh and dried Rose petals were found to contain higher amount of total phenolic calculated as mg/GAE/g. The amount was highest in ethanol >acetone >aqueous >chloroform extract. The ethanol extract of fresh and dried petals showed lowest IC50 values than the acetone extract by DPPH, ABTS, peroxynitrite inhibition, XOI, superoxide scavenging and lipid peroxidation inhibition methods. Conclusion: Ethanol extracts of fresh and dried Rose petals exhibited promising free radical scavenging activity. These extracts have good potential in the treatment of oxidative stress related disorders.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/28: Pharmacognostic evaluation of Mimusops elengi L. flowers: An important Ayurvedic medicinal plant

Deepti Gurjar, Sunita Shailajan

Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai, Maharashtra, India

E-mail: deepsgurjar@gmail.com

Background: Mimusops elengi L. flowers (Sapotaceae, Bakul) have been traditionally used in the history of medicine and Ayurveda as a brain tonic, in the treatment of asthma and for their different therapeutic activities like antiulcer, wound healing, antioxidant, etc. Objective: The present study was aimed to evaluate the pharmacognostic profile of M. elengi flowers. Materials and Methods: M. elengi flowers were subjected to evaluation of proximate and physicochemical parameters. Phytochemical characterization was carried out using validated HPTLC methods and gas chromatographyy–mass spectrometry (GC-MS) technique. Safety profile of the plant was evaluated by carrying out heavy metal analysis and acute oral toxicity study in rats as per OECD guidelines no. 420. Results: Proximate and physicochemical parameters of M. elengi flowers were established. HPTLC analysis of M. elengi flowers revealed the presence of some bioactive markers like β-sitosterol, lupeol and gallic acid whereas ursolic acid, quercetin and kaempferol were not detected. GC-MS analysis of flowers revealed the presence of another bioactive marker eugenol too along with presence of around 16 different compounds. M. elengi flowers showed the level of heavy metals (As, Pb, Cd and Hg) within the permissible limits. Results of the acute oral toxicity study ensures adequate safety margin of M. elengi flowers at 2000 mg/kg in rats. Conclusion: The pharmacognostic profile for M. elengi flowers obtained as a finding of this research work will contribute in their identification and standardization. The validated HPTLC methods for estimation of different bioactive marker can be applied to various other herbal drugs/ formulations for the estimation.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/29: Estrogenic effect of an Ayurvedic medicinal plant: Saraca asoca flowers

Gauri Swar, Sunita Shailajan

Herbal Research Lab, Ramnarain Ruia College, Matunga, Mumbai, Maharashtra, India

E-mail: gaurirswar@gmail.com

Background: Saraca asoca (Roxb). De Wilde. (Caesalpiniaceae), popular as Ashoka, is a medium evergreen tree regarded as one of the most important indigenous medicinal plants in India, which has been claimed for its potential use in women's’ health problems. Flowers are used in the treatment of diabetes, cancer, and hemorrhagic dysentery and are given as tonic to females with uterine disorders. Objective: The study was designed to investigate the estrogenic potential of standardized ethanolic extract of S. asoca flowers using ovariectomized female albino Wistar rat. Materials and Methods: Ethanolic extract of S. Asoca flowers has been standardized in terms of its kaempferol content using validated HPTLC method. Safety of the extract was evaluated at 2000 mg/kg body weight in female albino Wistar rats. Ovariectomized animals were administered with the extract at three dose levels - 100 mg/kg, 200 mg/kg and 400 mg/kg body weight orally once for two weeks. Estrogenic effect was studied by assaying the activities of various serum and plasma marker enzymes and hormones in addition to the effect on vaginal cornification, uterine histology, and uterotonic effect. Results: The kaempferol content was found to be 2.3 mg/g of extract. Estrogenic effect of standardized extract of S. Asoca was found to be in a dose-dependent manner. Conclusion: The standardized extract of S. asoca may be used as an alternative therapy for the treatment of female reproductive disorders caused due to estrogen deficiency.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/30: Evaluation of polyherbal oral formulation for antidiabetic activity

Vanita V. Gurale, Savita R. Kulkarni, P. K. Khatwani

Department of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Santacruz, Mumbai, Maharashtra, India

E-mail: vanita7827@gmail.com

Background: The individual extracts of leaves of Murraya koenigii (MK), Annona squamosa (AS) and roots of Plumbago zeylanica (PZ) had shown promising results in oral glucose tolerance test (OGTT) model. Objective: In order to study the potential synergistic activity of all these herbs, the capsules of each containing 200 mg extract of each of MK and AS and 40 mg of powder of PZ were prepared and tested for antidiabetic activity in STZ induced diabetic rat model. Materials and Methods: The aqueous extracts of all the ingredients that is, leaves of MK, AS, and root powder of PZ were prepared by hot decoction method and extracts were evaporated to get fine powder. The optimized dose revealed from OGTT study was selected for the capsule formulation. All the ingredients of the capsules were mixed together in required proportion with suitable excipients and filled into the capsules. The capsules were studied for their physicochemical stability, content of phytochemical marker by HPTLC and their antidiabetic activity using STZ induced diabetic rat model. Results: The OGTT study of individual extracts had revealed that at the dose of 200 mg for MK and AS, lowered blood glucose by 64%. The dose of the polyherbal formulation was calculated by extrapolation of these studies to human dose. The current study showed that the polyherbal formulation was 64% as effective as glibenclamide in STZ induced diabetic rat model. All the constituent herbs of capsule contain rutin that is known to have antidiabetic activity. Conclusion: Hence, a good oral polyherbal formulation is available for management of Diabetes.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/31: Enhancement of wound healing activity utilizing combination of napthoquinone dimer from Onosma echioides and Yashad bhasma

Purnima Vivek, Nikita Gawand, Chhaya Gadgoli

Department of Pharmacognosy, Saraswathi Vidya Bhavan's College of Pharmacy, Dombivli, Mumbai, Maharashtra, India

E-mail: purnimaviv@rediffmail.com

Background: Root bark of Onosma echioides is commonly utilized in folklore medicines to treat skin eruptions. It is rich in naphthoquinones, alkanins, and shikonins. Yashad bhasma is rich in zinc oxide is known as mild astringent, antiseptic and accelerates wound healing. Objective: The present study is aimed at preparation of a combination of isolated naphthoquinone dimer [C₄₀ H₄₄ O₁₀] with Yashad bhasma and to evaluate its wound healing potential. Materials and Methods: The isolated compound OE1, Yashad bhasma and combination of OE1 + Yashad bhasma were incorporated in simple ointment base in concentration 0.25%w/w, 1.25%w/w and 0.125%w/w + 0.62%w/w respectively. The above formulations were evaluated for the wound healing activity in rats using excision and incision wound models. Determination of parameters viz. period of epithelization and hydroxyproline content in Eschar in excision wound model while in incision wound model the tensile strength of new skin was determined. Results: The combination of OE1 and Yasahad bhasma (0.25%w/w + 0.62%w/w) showed to have better wound healing activity as significant (P < 0.001) reduction in the epithelization period and significant (P < 0.001) increase in hydroxyproline content and tensile strength than the isolated compound (0.25%w/w) and Yashad bhasma (1.25%w/w). Conclusion: Isolated compound OE1 when used in combination with Yashad bhasma exhibited better wound healing potential than OE1.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UP/OP/32: Effect of Pratisarniya kshara in the management of Gudabhramsha

Shikha Nayak, P. Hemantha Kumar

Department of Shalya Tantra, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: shikhanayak24@gmail.com

Background: The disease Gudabhramsha, which is described in Ayurvedic literature and rectal prolapse are one and the same disease with different terminology. Sushruta has described Kshara karma where Pratisaraneeya Kshara Karma is described in Arsha Chikitsa, in the context of ‘Bhrasta Guda’ (Rectal Prolapse), which is said to be the effective one. Objectives: To evaluate the effect of “Pratisaraneeya Kshara Karma” in the management of Gudabhramsha. Materials and Methods: Totally, 20 patients were randomly selected for Apamarga Pratisaraneeya Kshara Karma. During this treatment, the patients were observed for daily improvement and relative observations were noted on 1^st, 2^nd, 3^rd, and 7^th day. After that, patients were advised to come for follow-up once in a week, for 1 month and then once in a month for 6-months. All the cases were assessed as per the clinical criteria fixed for the study that is, degree of prolapse and anal sphincter tonicity. Results: In the present study, the improvement of degree of rectal prolapse showed that, on 1^st, 2^nd, 3^rd, and 7^th day, it was 91.4% improvement. But at the end of 1^st month, due to recurrence occurred in some cases the improvement in the degree of Prolapse was decreased from 91.4% to 87.23%. On 6^th month also it was 87.23%. However, 4^th degree rectal prolapse shows poor results in this study. Conclusion: After collecting all the research data, clinical profiles, reports, observations and results of this study, the efficacy of Pratisaraneeya Kshara Karma appears to be very effective in Gudabhramsha (Rectal Prolapse).

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/33: Assessment of state of aggravated Doshas through physical and bio-chemical examination of blood: A diagnostic study

Mukesh Bhayal, Naresh Kumar Mahawar, Reetu Sharma, Sisir Kumar Mandal, Surendra Kumar Sharma

Department of Roga Nidana Evam Vikriti Vijnana, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: mukeshbhayal@gmail.com

Background: Ayurveda is the clinical science of medicines that has holistic approach to improve the quality of life of an individual. The theory of tridosha is the foundation of Ayurveda. Doshas are not stable, they decrease, increase or remain same at every moment and they govern the physiological and physiochemical activities. Objective: To establish the correlation between the states of aggravated doshas and laboratory investigation. Materials and Methods: Total 99 subjects were selected randomly from OPD and IPD of our institute. The patients were examined clinically through questionnaires, and their physical examinations were recorded in a performa. Results: Doshik imbalance is the initial cause of any disease. Doshik imbalance reflects on physical, mental as well as bio-chemical character of the body. Low-density lipoprotein, triglyceride, very-low-density lipoprotein indicates aggravated kapha involved in diseases like prameha, medoroga, kaphaja hridroga etc., Blood urea level was slightly higher in pitta and vata prakriti indicating pitta is usually related to increased metabolic effect in the body. Conclusion: There are no objective parameters to assess the doshik state in the body, we usually assess doshik vriddhi and kshaya state based on clinical features.It was concluded that there are some variations in the bio-chemical factors that may help to assess the condition of doshas very easily and accurately.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/34: Multiple lipomatosis and its Ayurvedic management

Pranav P. Redkar, Yunus G. Solanki

Department of Shalya Tantra, Government Ayurved College, Osmanabad, Maharashtra, India

E-mail: drpranav213@gmail.com

Background: Lipomatosis is a rare disorder of unknown etiology in which multiple lipomas are present on the body. Many discrete, encapsulated lipomas are formed on the trunk and extremities, with relatively few appearing on head and shoulders and not usually seen on face, scalp, plantar and palmar surface. Lipomas are usually observed between third to fifth decades of life. Existing Lipomas keep on growing in size and newer lipomas keep on appearing, disfiguring the body parts. In Ayurveda this condition is known as Medoj granthi and it is produced as a result of vitiation of Meda dhatu. There is no known remedy for multiple lipomatosis in contemporary that is, modern medicine except surgical removal that is inadequate. Objective: To assess the effect of Ayurvedic remedy in the management of multiple lipomatosis. Materials and Methods: In the present study, seven patients of multiple lipomatosis were treated with Ayurvedic medicine. Detail family history was taken, and family members assessed for multiple lipomatosis. Patients were thoroughly investigated. According to prakriti and rutu, vidhipurvak shodhan chikitsa followed by Shaman Chikitsa comprising of trikatu churna, kanchanar guggul and medohar gana kashaya of Charaka samhita was given for 6 months. Follow up was taken after every 15 days for 3 months and thereafter every month for next 3 months. Result: As a result of above treatment, the growth of existing lipoma and appearance of new lipomas was arrested. Conclusion: Ayurvedic formulations followed by Shodhan chikitsa is effective in multiple lipomatosis and should be persuade for a longer time.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/35: Role of Kushthadi lepa and Guduchi churna in the management of Vicharchika

Saroj Choudhary, Chetana Agrawal, Sisir Kumar Mandal, Surendra Kumar Sharma, S. K. Khandal

Department of Roga Nidan and Vikriti Vijnana, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: sarojchaudhary@gmail.com

Background: Vicharchika is described under Kshudra kushtha in Ayurvedic classics, also mentioned as a curable disease yet the relapsing nature of this disease makes it much harassment for patient and also troublesome for physician. The disease Vicharchika is not a life-threatening one, but makes patient upset due to its appearance. Objective: To find out the role of Kushthadi Lepa and Guduchi Churna (Tinospora cordifolia Thunb. Miers) in the management of Vicharchika. Materials and Methods: For this study, total 30 patients of age group 18 to 70 years were selected and were equally categorized into three groups. Group A patients were given Lepa Q. S and Guduchi Churna 5 g twice a day after Samsodhana karma (Virechana karma). Group B patients were advised for Lepa along with Guduchi churna. Group C patients were advised for Lepa only. The duration of the trial was 30 days. Result: Highly significant relief was seen in all the parameters of Groups A, B and C respectively that is, marked improvement in erythema (60%, 47.6%, 55%), Pidika (68.4%, 58%, 54%), pruritus (56.52%, 59%, 62%), oozing (66%, 83%, 62%). Although the relief was seen, it was not statistically significant. Conclusion: Virechana Karma provides comparatively better results in symptoms of Vicharchika like Shotha, Svayathu, Kandu and Pidika. The trial drug Guduchi acted as an immunomodulator to combat the disease.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/36: Formulation development of an anti-inflammatory herbal transdermal film

Chintan Bhavsar, Trusha Parekh, Anjali Gohel, Tabassum Khan

SVKM's Dr. Bhanuben Nanavati College of Pharmacy, Mumbai, India

Email: chintanbhavsar93@gmail.com

Background: The conventional oral anti-inflammatory drugs available today are associated with side effects like gastric irritation thereby reducing the patient compliance for chronic use. Herbal medicines are believed to be relatively safe and effective. Hence, an anti-inflammatory herbal transdermal film was formulated. Objective: To formulate an herbal transdermal film containing anti-inflammatory extract. Materials and methods: The Methanol extract of Acacia nilotica (F: Mimocaceae) pods (MEA) exhibited a significant in vivo anti-inflammatory activity in rats and it also showed a significant anti-arthritic activity in our laboratory studies. Also, Cinnamon oil (Cinnamon zeylanicum) and Lemon oil (Citrus limon) were incorporated into the formulation for synergistic activity. 1g each of lemon oil and cinnamon oil was emulsified in glycerin using Tween80 as emulsifier. Polyvinyl pyrrolidone and polyvinyl alcohol were dissolved in adequate hot distilled water. 10% MEA in DMSO and the emulsion prepared was added to the above aqueous solution and uniformly stirred. This solution was poured in petri plates and allowed to dry to form the film. Physicochemical evaluation tests and preliminary diffusion studies were carried out using Franz diffusion cell and the results were obtained. Results: The film obtained was uniform in terms of weight, flatness, percent moisture loss and thickness. Preliminary diffusion studies that were carried out showed satisfactory drug release through the Nylon 6.6- 0.45μ lipid membrane. Conclusion: The herbal transdermal film was prepared successfully. This film can be used as an alternative to the oral NSAIDs for chronic treatment of arthritis.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/37: A randomized controlled trial of Apamarga (Achyranthus aspera L.) and Vyoshadi Guggulu on Sthaulya (Overweight)

Manisha Talekar¹, Pawankumar Godatwar¹, Sisir Kumar Mandal¹, Sudhir Turi²

Department of Roga and Vikriti Vijnana, National Institute of Ayurveda, Jaipur, Rajasthan, ²SSMB Ayu College, Moga, India

Email: dr.mani21jan@gmail.com

Background: Obesity is one of the highly neglected health problems and a type of which invites many major and minor diseases. It certainly shortens the life-span. Presently obesity has turned in to a pandemic and needs attention from all aspects of its control. Objective: The Present study was done to assess the efficacy of trial drug Apamarga Tandula and Vyoshadi Guggulu in comparison to standard drug Sibutramine on Anthropometric Parameters. Materials and Methods: 60 subjects were randomly divided in three groups. Apamarga Tandula was given to group A, Apamarga Tandula and Vyoshadi Guggulu was given to group B and the standard drug Sibutramine was given to group C. The subjects were assessed every 15 days- before start, once during and at the end of trial of 8 weeks. Results: Apamarga Tandula showed significant improvement in body fat %, Waist circumference and SKFT while Apamarga Tandula and Vyoshadi Guggulu showed significant improvement in BMR, Waist circumference and SKFT. The control drug sibutramine showed significant improvement in body fat % and Waist circumference. Conclusion: When the adverse affects of the control drug sibutramine are taken into account, it can be safely concluded that the Apamarga Tandula is a better drug for the management of obesity especially in combination with Vyoshadi guggulu than the control drug sibutramine.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/38: A clinical study of changes in the lipid profile after administration of Amrutadhya Guggulu w.s.r. to hyperlipidemia

Barapatre Pranali S, Wankhade VH

Department of Kaychikitsa, Vidarbha Ayurved Mahavidyalaya, Amravati, India

Email: pbarapatre317@gmail.com

Background: Hyperlipidemia is one of the major lifestyle disorders. It has been identified as a potential risk factor in the manifestation of serious diseases like Ischemic Heart Disease (IHD), Diabetes, Stroke etc., which is an important cause of morbidity and mortality all over the world. These lifestyle disorders have been referred to as the Santarpanjanya Vyadhis in the classical texts. As Guggulu is most potent drug in Hyperlipidemia also proven its Karshan property, Guggulu compound i. e. Amrutadhya Guggulu as indicated in Sthoulya Chikitsa by Aacharya Chakradatta was selected for the clinical study. Objectives: To evaluate the clinical efficacy of Amrutadhya guggulu and to study the changes in lipid profile after administration of Amrutadhya guggulu in Hyperlipidemia. Material and Methods: 60 clinically diagnosed patients attending the OPD/IPD of our institute were selected for the present study randomly and divided in two groups. Group A contained 30 patients to which Amrutadhya Guggulu 500mg TDS was given and Group B contained 30 patients which were on placebo. Duration of treatment was 60 days; follow-up was taken every 15 days. Result: The result of the study revealed that Amrutadhya guggulu was found to have a significant effect in the lipid profile. Conclusion: Amrutadhya guggulu proved effective in treating Hyperlipidemia.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/39: Virechana karma-A statistical analysis

Rahul Singh Sisodia, B.S.Prasad

KLE University's Shri BMK Ayurveda Mahavidyalaya, Belagaum, India

Email: dr.rahulsingh0508@gmail.com

Background: Panchakarma is a set of five procedures claimed to eliminate the vitiated doshas from the body and Virechana is one of them. For Virechana karma kostha assessment is necessary and Virechana karma is administered with different Virechaka yogas such as Trivrita Lehya, Dantyavaleha, Abhayadi Modaka etc. Objective: To assess the efficacy of Trivrita Lehya on types of kosthas. Materials and Methods: In KLE Ayurveda Hospital, Belgaum total number of virechana administered from January 2012 to September 2014 is 753. All Virechana karmas were selected to carry out retrospective study irrespective of disease. Out of them 409 patients (54.31%) were having krura kostha, 126 (16.73%) madhyama kostha and 218 patients (28.95%) Mridu Kostha. Out of them 621 Patients (82.47%) were administered with Trivrita lehya and rest 132 patients (17.52%) administered with other Virechaka Yogas. In krura kosthi patients 30 gm Trivrita Leha is given to 291 (71.14%), in Madhyama kosthi 92 patients (73.01%) 25 gm Trivrita Leha given, in Mridu Koshthi 189 (86.69%) 20 gm of Trivrita Leha given. Results: By Trivrita lehya madhyama, avara, pravara shuddhi observed in krura, madhyama, mridu kostha respectively. Conclusion: If kostha assessment and dose calculation is perfect then Trivrita leha can give good results in Virechana.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/40: Handshake of Ayurveda with modern science: Novel approach towards development of antimicrobial skin formulations

Divij Kotian, Rujuta Deshpande, Shubha Govil, Aparna Deshmukh

Thakur College of Science and Commerce, Mumbai, India

Email: drasdeshmukh@gmail.com

Background: Modern antimicrobial formulations are focused to act on microbes, and not the hosts. Ayurveda prescribes herbal formulations indicated for multiple skin conditions and also for improving host immune mechanisms. Benefits of nanoparticles in antimicrobial formations are already reported. This prompted us to explore in vitro the novel approach of combining modern scientific tools with ancient wisdom of Ayurveda as a step towards development of antimicrobial formulations. Objective: The study aimed to evaluate in vitro anti-microbial and immuno-modulatory activity of various herbs indicated in various skin infections, ZnO nanoparticles and their combinations. Materials and Methods: 23 herbal preparations of herbs, ZnO nanoparticles and their combinations were tested for antimicrobial effect against pathological isolates of Staphylococcus aureus and Candida albicans using agar cup method. Their effects on the first line of host defense were studied by determination of neutrophil phagocytic activity. Results: Rubia cordifolia (Manjishtha), Allium sativum (Garlic), Piper betle (Beetle), Ocimum sanctum (Tulsi), Terminalia chebula (Harade), Azadirachta indica (Neem) and Ficus bengalensis (vat) were effective against Staphylococcus aureus and Candida albicans. Maximum stimulation of neutrophil phagocytosis was observed with Rubia cordifolia (% phagocytosis 86.6 vs. control 37 ± 10 and phagocytic index 1.66 vs. control 1.3 ± 0.2) and Ocimum sanctum (phagocytic index 1.95 and % phagocytosis 65.4). ZnO nanoparticles in the range of 0.1- 0.5 mg/ml showed antimicrobial activity and also stimulation of phagocytosis (% phagocytosis 87.5; PI: 1.69). Conclusion: Amongst the tested combinations, additive but not synergistic antimicrobial action was observed between ZnO nanoparticles and Manjishtha decoction.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/41: Development and physico-chemical evaluation of pain balm ointment by Gunja seeds

Prashant Kumar Singh SantoshS. B, K.S. Gudaganatti, R.R.Hiremath

KLEUs BMK Ayurveda Mahavidyalaya Shahapur Belgaum, India

Email: singh9947@yahoo.com

Background: Gunja (Abrusprecatous) is an herbal poisonous drug mentioned under the group of upavishas in Ayurveda. External application of paste of Gunja seeds have said to be useful in the management of pain. Objective: To develop an ointment by using extract of Gunja seeds and other bases by fusion method. Materials and Methods: Soxhlet extraction of Gunja seeds was prepared by using ethyl alcohol. The ointment base was prepared using fusion method by using hard paraffin 370 C 5%, Cetostearyl alcohol 48-560 C 5%, Lanolin 370 C 8%, Bees wax 62-640 C 10%, Soft Paraffin 370 C 72%. The ratio of base and extract was base 90% and extract of Gunja seeds 10%. Soon after cooling the ointment was stored in air tight container. Results: Physicochemical results of Gunja seeds showed foreign matter 1%, Total ash 2.75%w/w, Acid insoluble ash 0.37%w/w, Water soluble ash 0.74%w/w, Alcohol soluble extracts 12.8%, Water soluble extracts 19.6%, pH 6.15. HPTLC Analysis of ethanolic extract of Gunja showed 5 bands at 245nm with Rf values viz. 0.06, 0.19, 0.24, 0.29, 0.44. And showed 7 bands at 366nm with Rf values viz. 0.03, 0.07, 0.35, 0.42, 0.48, 0.56, 0.67. The analytical results of the Gunja Ointment is pH 5.9 2, Spread ability 86.8 Sec/G/Cm, MLT Negative, Viscosity is 5120mPas. Conclusion: Prepared ointment showed agreeable organoleptic characteristics and it passes all the analytical tests as conducted for semisolid dosage forms, along with it showed good stability for the period of 8 months at room temperature.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/42: Treadmill stress test as a measure of Deha bala w.s.r. to Pandu roga

Ashish Reja¹, Pawankumar Godatwar¹, Vinod Chandra Singh¹, Sevatkar Balkrishan¹, Sisir Kumar Mandal²

Department of Roga nidana evam Vikriti vijnana, N.I.A. Jaipur, Rajasthan, ²Durga Ayurveda College, Raipur, Rajasthan, India

Email: dr.ashishreja@gmail.com

Background: Deha-bala implies physical capability of an individual. The characteristics of deha-bala are Utsahalakshana when one can perform work in unfavorable conditions also and Upachayalakshana can be understood by body built up, stability and compactness of the body. Objective: The Present study was done to evaluate Treadmill Stress Test as a measure of Deha-bala in Pandu roga Materials and Methods: 60 subjects of Pandu roga were recruited for the study after written informed consent and randomly divided in two groups. Balya churna was given to group A and Nisha loha vati was given to group B. Deha-bala was measured with help of Treadmill Stress Test which was Bruce protocol (Dr. Robert. A. Bruce, 1963). Results: A significant increase in deha-bala was found in group A from (2.10 to 3.30), while in group B it increased from (2.07 to 3.23). Conclusion: It can be concluded that Treadmill Stress Test was an efficient objective for measuring the Deha-bala status.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/43: Evaluation and standardization of Arogyavardhini Vati

Rohini Waghamare, Pratima Tatke

Department of Pharmaceutical Chemistry, C.U. Shah College of Pharmacy, S.N.D.T. Women's University, Santacruz, Maharashtra, India

Email: rohini.chandratre@gmail.com

Background: Arogyavardhini Vati is an ancient well known Ayurvedic formulation. It is widely used in the treatment in the skin disorders, fever and problems related to digestive system. The Vati consists of different crude drugs such as Kutaki, Haritaki, Bibhtaka, Amalaki, Eranda, Guggulu, Neemleaves, Shilajatushudhhaand Neemjuice and the metal and metal preparations such as purified mercury, purified sulfur, lauhabhasma, abhrakbhasma, tamrabhasma. However the systematic evaluation of this formulation is yet not reported and published which is essential for its world-wide acceptance. Objective: To evaluate and standardize Arogyavardhini Vati as per Ayurvedic Pharmacopoeia of India. Methodology: Macroscopic and microscopic evaluation of the Arogyavardhini Vati is carried out by pharmacopoeial methods. The other parameters such as, weight variation, size, hardness, disintegration time, friability, heavy metal analysisare also evaluated and standardized as per Ayurvedic Pharmacopoeia of India. HPLC profiles/HPTLC finger prints have been developed. Results: Microscopic evaluation confirmed the presence of the crude drugs ingredients. The HPLC profile/HPTLC finger prints reports the marker compound from the crude drug in the formula. The other pharmacopoeial parameters are within the limits standards specified in Ayurvedic Pharmacopoeia of India. Conclusion: The study evaluates and compares Arogyavardhini Vati of two different manufacturers. This study will set the guidelines for quality control of Arogyavardhini Vati.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/44: Comparative in vitro Anthelmintic activity of two ayurvedic herbal drugs

Kulkarni Aniruddha Shreedhar¹, Kulkarni Anuja Aniruddha², Khot Vidyarani Sujitkumar³

¹Vaidyaraj Datar Panchabhautik Chikitsa and Samshodhan Kendra, Sangli, ²Department of Hospital and clinical pharmacy, Dr. J. J. Magdum Pharmacy College, Jaysingpur, ³Department of Pharmacology and Toxicology, Dr. J. J. Magdum Pharmacy College, Jaysingpur, India

Email: askulkarni2@gmail.com

Background: The World Health Organization (WHO) estimates that infection with round worm, whipworm and hookworms with associated morbidity, affect approximately 250 million, 46 million and 151 million people, respectively. So, worm infestation as a public health problem needs immediate attention. Sadly, very few drugs are used for deworming specially in children. Objective: The main objective of the present study was to compare in vitro anthelmintic activity of two herbal ayurvedic drugs namely Kapila and Chaturthak yoga, used as vermicides along with Albendazole as a standard reference. Materials and Methods: The two Ayurvedic herbal drugs presented in this study are Kapila and Chaturthaka yoga. The aqueous and methanolic extracts of these drugs were prepared using maceration, soxhlet apparatus and thermostatic bath. These extracts were investigated for anthelmintic activity using earthworms (Pherentima posthuma). Albendazole was used as a reference standard at 10 mg/ml. Results: Aqueous and methanolic extracts of these two drugs at different concentrations (10, 20 and 40mg/ml) demonstrated dose dependent vermicidal activities. Aqueous extract of Kapila at 40 mg/ml caused paralysis and death at 18.83 and 36.5 minutes; while it's methanolic extract showed paralysis and death at 21.33 and 46.66 minutes respectively. Aqueous extract of Chaturthaka yoga at 40 mg/ml caused paralysis and death at 10 and 18.83 minutes; while it's methanolic extract showed paralysis and death at 6.16 and 19.33 minutes respectively. Conclusion: Both the drugs possess potent anthelmintic activity when compared to Albendazole. Chaturthaka yoga is the most effective drug.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/45: Anti secretary and anti ulcer activity of Yashtimadhu Ghrita and Pittantak Yog- An experimental study

Varsha Mundhra, Jagadeesh G. Mitti, K. Shankar Rao

National Institute of Ayurveda, Jaipur, India

Email: dr.varshamundhra@gmail.com

Background: The science of Ayurveda, directs towards the different secretions from gastrum and liver as pitta. Abnormal secretions may leads to acid peptic disorders (APD) which can be correlated with diseases viz., Amlapitta, Annadrava shool and Parinaam shool. These may lead to hematemesis, melena, and perforation if failed to treat them in time. Objective: To evaluate the gastric anti secretary and anti ulcer activity of Yastimadhu Ghrita and to find out the effective formulation among the two selected formulations. Materials and Methods: Yashtimadhu ghrita (anubhoot yog) and Pittantak Yog (NIA pharmacopeia) were taken for the study. Yashtimadhu ghrita and Pittantak yog were compared experimentally with Ranitidine (standard drug) for anti-secretory and anti-ulcer activity in albino rats weighing 150-200 gm. in four groups (six rats in each). Results: The ulcer index in Control, Standard, Piitantaka yoga and Yastimadhu ghrita group was 6.3, 0.85, 0.9 ± 0.2, 1.3 ± 0.28 and 1.5 ± 0.22. The results were statistically significant by’t’ test. Conclusion: The results found were significant and equipotent with each other.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/46: Concept of Oja vis-a-vis endoplasmic reticular function: an approach to decode the holistic view

Anagha Ranade, Rabinarayan Acharya

Department of Dravyaguna, IPGT and RA, G.A.U, Jamnagar, India

Email: anagharanade11@gmail.com

Background: In Ayurveda, Oja, being one of the most distinctive concepts is a factor of prime importance related to vitality. Ayurveda depicts the definition, types (Para, Apara); site (Hridaya- Heart, sarvashariravyapi – presence in cellular level), quantity (vindu - in drops), function (bala-vitality) and its derangement etiology etc., of Oja. Various researchers based on their own ideas have given its modern parlance with immunity and stem cell etc. Objective: To decipher the fundamental concept of Oja vis-à-vis endoplasmic reticular function. Material and Methods: A critical analysis of information on Oja from available Ayurvedic literature and endoplasmic reticular function form modern Physiology texts. Result: ‘Endoplasmic reticulum (ER)’ is a multifunctional membranous organelle forming a network in the whole body and is responsible for lipid biosynthesis, calcium storage and protein folding and processing. Etiological factors of Ojadushti (dysfunction) are (Abhighata-may be environmental stress), (shoka-mental stress), (shrama-physical stress, etc) which share similarity with stressors that disrupt function of ER ultimately leading to ER stress causing lifestyle disorders. Achieving cellular homeostasis is now being emphasized in the management of various diseases, in conventional medicine. The cellular homeostasis is dependent largely on proper folding of proteins where as in proper folding results into accumulation of misfolded proteins ultimately enhancing cellular death. Scientific reports on vayathapana herbs like Amalaki, Punarnava, Guduchi etc., to alleviate ER stress can be give an evidence to correlate the ER with Oja. Conclusion: The present hypothesis gives an insight to decipher a novel concept of Oja and its relation with Endoplasmic reticulum.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/47: Pharmacognostical evaluation of Ipomoea pentaphylla Linn

Minutee Patel, Rajesh Mer, Preeti Pandiya, Anagha Ranade, RN Acharya

Department of Dravyguna, IPGT and RA, GAU, Jamnagar, India

E-mail: drrnacharya@gmail.com

Background: In the present era, the genuine identification of crude drug is posing a problem to the physicians and pharmaceuticals. Medicinal plants used in Ayurveda are often found to be adulterated and substituted which decreases the efficacy of the drugs in practice. Pharmacognosy is a prime tool of basic authentication of medicinal plants in order to standardize them. Ipomoea pentaphylla Linn, a tribal claimed medicinal plant, not scientifically evaluated and reported till yet, was assessed for its basic pharmacognostical characters. Objective: To evaluate pharmacognostical parameters of whole plant of I. pentaphylla. Material and Methods: The fresh plant was collected, identified, authenticated and assessed for its macroscopy followed by microscopy of leaf, petiole and seeds. The powder microscopy, histo-chemical study and stomatal index were also studied following the standard procedures given in authentic text and the measurements of specific characters were noted and microphotographs were recorded. Results: The morphological characters of I. pentaphylla strongly support the Convolvulaceae family. The microscopic characters of root, stem, leaf, petiole, and seed showed presence of cortex, epidermis, parenchymatous tissue and vascular bundle along with presence of cluster, rosette and prismatic crystals, trichomes, starch grains, etc. The seed mainly showed presence of mucilage containing gland. The stomatal index was 20 (upper surface) and 20 (lower surface). Powder microscopy revealed presence of simple and compound starch grains, cluster and rosette crystals of calcium oxalate, oil globules, and paracytic stomata. Conclusion: The present pharmacognostical observations may be considered as a standard parameter for basic identification of I. pentaphylla linn.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/48: Clinical Study on the effect of Bringaraj Punarnava Ghana Vati on Uccharaktachapa with special reference to essential hypertension

Anupama Mahadeorao Bathe, Ajay Kumar Sharma, Ajay Kumar Sahu

Department of Kayachikitsa, National Institute of Ayurveda, Jaipur, Rajasthan, India

Email: dranupamabathe@gmail.com

Background: Hypertension is asymptomatic in nature but produces dreadful effects on the body. So it is called as silent or hidden killer. Due to its high prevalence Indian Council of Medical Research (ICMR) and All India Institute of Medical Sciences (AIIMS) have declared India as a “Nation of Hypertension”. Whole world is looking for the Ayurvedic management of the same. Objective: To study the efficacy of Bhringaraj Punarnava Ghanvati in Uccharaktachapa (Essential-hypertension). Material and Method: 15 patients were selected randomly from Arogyashala OPD and IPD, of our institute, which were well diagnosed of Uccharaktachapa/Essential Hypertension 1 and II grade as per 7^th JNC and WHO criteria for Diagnosis Hypertension and symptoms described in Ayurvedic classical text. Bringraj-Punarnava ghana vati was given in dose of 2 tab. (each 500 mg.) three times in a day with lukewarm water for 45 days and IPD subjects were assessed routinely while OPD in 7 days follow up. Results: Bringraj-Punarnava ghana vati showed highly significant result in both the systolic and diastolic blood pressure and significant improvement in subjective parameters shirashool, bhrama, klama, hritspandan and anidra. Conclusion: Therapy in the form of administration of Bhringraj Punarnava ghan vati is safe and effective in the management of Uccharaktachapa (Essential Hypertension).

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/49: Can In Silico Study Shift The Paradigms In Ayurveda Drug Research Leading To Prakruti Based Medication?

Harshad Satish Rasane, Ravindra P. Bhurke

Department of Kriya Sharir, R.A. Podar Medical College (Ayu.), Worli, Mumbai, Maharashtra, India

Email: drrasane@gmail.com

Ayurveda advices the use of various medicinal herbs in treatment as well as in day to day activity. This conceptual application directly or indirectly tries to maintain the body's equilibrium. The proper knowledge of such equilibrium is best available under the concept of Prakruti. The genetic and the genomic study can be correlated with the concept of Prakruti. The boom in the field of genome has led to existence of huge magnitude of data which is better maintained, utilized and applied with the help of bioinformatics. The in-depth knowledge of Prakruti and in silico study with simultaneous aid of bioinformatics can be beneficial and lead to development of Prakruti based medicine.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/50: Validation of Retopareeksha vis-à-vis semen analysis

Sukumar Nandigoudar, Anju Sharma¹

Department of Kayachikitsa, KLEU's Shrí BMK Ayurved Mahavidyalaya, PG Studies and Research Centre, Belgaum, Karnataka, ¹Department of Svasthavritta, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: dr.annu1802@gmail.com

Background: Śukra is considered as supreme dhatu and its dushti leads to male infertility and sexual dysfunction. Retopareeksha is a source to understand various Śukra dushti. Semen analysis is one of the important diagnostic tools in the management of male infertility. Objectives: To understand and validate retopareeksha as a standard Ayurvedic diagnostic tools in male infertility. Materials and Methods: Charaka and sushruta samhita are the literary resource to assess Shudha śukra (healthy) and śukra dushti (morbid semen). The qualities of shudha and dushta śukra will be understood and assessed with the help of modern seminal parameters. To assess the parameters of Retopareeksha modern instruments like phase contrast microscope, Rohem's sperm counting chamber, pH measuring strips, calibrated pipette etc., are used and eosin stains to examine the viability of sperms. Results: Abnormal physical characteristics of the semen can be understood by the doshic involvement. Phenila, Tanu, Ruksa represents vataja sukradushti; Vivarna (neela/peeta/ashveta) indicate pittaja śukradushti; Atyarta picchila denotes Kapha dosha; Putipuya indicate pittakapha involvement and anyadhatu samsrishti is due to shonitadi dhatu. Conclusion: Retopareeksha focus on panchendriya pareeksha to assess physical characters and morbid dosha of semen. Phalavattata, a main character may be attributed to viability, motility, count and normal morphology of sperms. The sperm endowed with normalcy of all these is able to fertilize the ovum that is, Garbhakara. Retopareeksha with the aid of modern tools gives exact idea about the pathology involved and thus helps in effective management.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/51: An Ayurvedic approach in the management of neurocysticercosis: A case study

Suraj Rathod, Amit Nakanekar, Sunanda Bhopale, Ajit Devikar, J. D. Gulhane

Government Ayurved College, Nagpur, Maharashtra, India

E-mail: suraj_rathod78@yahoo.com

Background: Neurocysticercosis is infection of the brain by the larval form of the pork tapeworm taenia solium. Neurocysticercosis is form of cysticercosis and is the most common infection of central nervous system. In 80% cases symptoms are seizures, headache, visual disturbance, nausea, abdominal cramps and diarrhea. Among the rare incidences some abnormal symptoms occur such as dysarthria, extraocular movement palsy, hemiplegia, movement disorders, and gait disturbances. Objective: To see the effect of Krimi treatment in a case of neurocysticercosis. Materials and Methods: A 49-year-old male patient came to out-patient department of our institute with complaints of weakness in left side of upper and lower limb, slurred speech, difficulty in walking since 10 days and having H/O seizures 30 years ago. Patient was diagnosed as a case of neurocysticercosis through computed tomography (CT) brain, cerebrospinal fluid, and other hematological investigations. Patient was admitted and treated with Ayurvedic principle of krimi management. Results: Patient showed complete recovery in clinical assessments in terms of speech, gait weakness and CT scan. Conclusion: Ayurvedic krimi treatment is effective in the management of neurocysticercosis. However, larger studies are required.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/52: A novel dosage form for enhancement of bioavailability of herbal topical formulations

Sonali R. Mali, Jyotsna M. Taklikar, Rupali A. Mali¹

Department of Rasashastra and Bhaishajya Kalpana, Loknete Rajarambapu Patil Ayurvedic Medical College, Post Graduation and Research Center, Islampur, ¹Department of Pharmaceutical Chemistry, Pravara Rural College of Pharmacy, Pravaranagar, Maharashtra, India

E-mail: dr.sonalimali@gmail.com.

Ayurveda is a life science, which explains about the different dosage forms. Topical delivery systems for drugs make localized administration of the drug anywhere in the body through ophthalmic, vaginal, skin and rectal routes. Herbal medicine has become an item of global importance both medicinal and economical. Although the usage of these herbal medicines has increased, their quality, safety and efficiency are serious concerns in industrialized and developing countries. Hence, turning to safe, effective and time tested Ayurvedic herbal drug formulation would be a preferable option for the better patient acceptance. Recently, pharmaceutical scientists have shifted their focus to designing a drug delivery system for herbal medicines using a scientific approach. For a long-time herbal medicines were not considered for development as novel formulations owing to lack of scientific justification and processing difficulties in complex polyherbal systems. Novel drug delivery system in the field of medicine had taken a popular attention as it makes the intake, bioavaibility and overall therapeutics of drug easier and in a short period of time. An added complication is that both the traditional and modern methods of preparation of herbs are sometimes selected on the basis of technologically feasible, convenient, or affordable, rather than might be ideal from the consideration of traditional practices or clinical efficacy. Considering these facts that review is an attempt to present a concise profile of different traditional topical herbal formulations includes lepa, malahara, upanaha, creams, ointments, pastes and novel topical formulations like oragnogel, emulgel, ampiphilic gel and transdermal patch.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/53: Epidemiological survey of Pranvaha srotodusti w.s.r. to Vayudusti (air pollution)

Vidya Kumari Meena, Birendra Kumar¹, Surendra Kumar Sharma, Piyush S. Mehta

Department of Roga and Vikriti Vijnana, National Institute of Ayurveda, Jaipur, Rajasthan. E-mail: drmeenavidya@gmail.com

Background: In the present era, due to modern civilization, urbanization, and industrialization anthropogenic sources are becoming most responsible for the air pollution. Transport, power plants, oil refining, burning crop waste, fumes from paints varnish, aerosol sprays, nuclear weapons, toxic gases, etc., are main anthropogenic sources responsible for air pollution Objective: This study was done to find out the prevalence of pranvaha sroto dusti in general population of Jaipur. Materials and Methods: For epidemiological study, total 500 subjects were surveyed from 5 different places of Jaipur by camping. Total five groups were made (100 subjects per group). They were assessed on the basis of subjective parameters lakshans of pranvaha sroto dusti (swasa, kasa, hikka and pratishyay) and also on the basis of peak expiratory flow rate recorded by peak flow meter as objective parameters. Results: This study showed that prevalence of pranvaha sroto dusti was more in overweight persons, short stature persons and smokers. Swas and kasa are the main symptoms, which persists for long-time in those who were exposed for a longer period in that particular polluted atmosphere. Conclusions: Based on this study, it may be concluded that pranvaha srotasa is directly connected with the ambient air (pran vayu) so as the air becomes polluted, the pranvaha srotasa also becomes vitiate and different kinds of diseases begin to start in that particular srota.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/54: Physicochemical study of different methods of Gandhaka Shodhana and evaluation of its antimicrobial property

Shraddha R. Waghmare, Shymal Nirmal

Department of Rasashastra, Ashwi Rural Ayurved College, Ahmednagar, Maharashtra, India

E-mail: shraddhawaghmare1989@gmail.com

Background: Gandhak is one of the uprasa mentioned in rasashasatra. It has been given prime importance in rasashasatra, because of its rasayana properties. It is also mentioned in Ayurvedic texts that gandhaka is krumighna. This property is considered the main section of the desired study. Micro-organisms selected for the study will be accessed by the antimicrobial property of three purified gandhaka samples obtained after three different types of shodhana vidhis. Objectives: Physicochemical study of different methods of gandhaka shodhana and to evaluate the evaluation its antimicrobial property. To carry out the shodhana of gandhaka by different methods, to carry out the physicochemical analysis of samples prepared and to find out the antimicrobial property of prepared samples. Materials and Methods: Material which was required for the shodhana method was procured from local market. For analytical study and antimicrobial study, material available in the laboratory was used. Gandhaka purification was done by Goghrit and Godugdha, Bhrungraj swarasa and Til Tail. Physicochemical analysis was done before and after purification of Gandhaka. Antimicrobial study was done by well diffusion assay. Results: There was a remarkable difference observed in antimicrobial activity of raw and shodhita samples of Gandhaka by different methods. Conclusion: This study concluded that there was the difference observed between raw and shodhita samples.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/55: The technical development of Bahyaswedana (external sudation) Karma w.s.r Sthanika (Kati, Greeva, Prusta, Janu and Hrud) Basti

Umesh S. Holennavar, B. S. Prasad, R. V. Sanapeti

Department of Panchakarma, KLEUs Shri BMK Ayurved Mahavidyalaya, Belgaum, Karnataka, India

E-mail: Umesh.holennavar@gmail.com

Background: Ayurveda is an indigenous system of medicine practicing in Indian sub-continent since ages. It has a special mode and modalities of drug administration called as Panchakarma and some modified pre Panchakarma procedure Bahyaswedana like sthanika basti-Kati basti and Jaanubasti. These procedures are costly and required huge amount of oil to perform and also time consumption hence modification of these technique with modern technique hence present concept has been put forth. Objective: Implementation of new technology in external sudation therapy with view of giving good effect of therapy. Materials and Methods: The Shanikabasti has main procedural component of constant maintenance of temperature this can be performed by adopting near-infrared (NIR) light along with oil. Moreover, NIR light has a special property of analgesic thus modified procedure can become worth and cost-effective procure. Results: The NIR light has analgesic, wound healing, and tissue reforming capacity. The NIR disassociates nitric oxide leads to restoration of adenosine triphosphate production, a reduction of oxidative stress and subsequently reduction in inflammation. This can enhance the effect of Sthanikabasti by performing with oil. This procedure may have few limitations like selective hypersensitivity to NIR light. Conclusion: Adaptation of modern technique in Ayurvedic science is a need of the hour in Ayurveda. Present procedure is one such innovative thought, and this can surely cost-effective and much more effective than normal Sthanikabasti technique.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/56: The ocular hypotensive effect of Gokshuradi guggul in primary open angle glaucoma: A Pilot Study

A. Patil Ashwini

Department of Shalakyatantra, Dr. D Y Patil College of Ayurved and Research Institute, Nerul Navi Mumbai, Maharashtra, India

E-mail: drashwinipatil14@gmail.com

Background: Glaucoma is a group of eye diseases that damages the optic nerve, which carries information from the eye to the brain. Glaucoma is estimated to affect 60.5 million persons worldwide by the year 2010. The estimated prevalence of glaucoma for India is 11.9 million. Glaucoma damage vision so gradually that nobody notice it until the disease is at an advanced stage. Either accidentally or in the advanced stage, glaucoma is diagnosed. This can permanently damage vision in the affected eye (s) and leads to blindness if left untreated. It is normally associated with increased intraocular pressure (IOP). Primary open-angle glaucoma (POAG) is one of the most common causes of irreversible blindness. The progressive nature of glaucoma and its growing incidence makes its therapy an important target for research. Herein, we evaluate the effect of Gokshuradi guggul on IOP in eyes with POAG. Objectives: To evaluate the ocular hypotensive effect of Gokshuradi guggul in POAG. Materials and Methods: Patients in Shalakyatantra out-patient department and in-patient department were recruited in this study after proper consent. Subjects in this study were treated with Gokshuradi guggul 250 mg 2 bd – 4 weeks. Followed by 2 hs for 8 weeks. Twenty-four eyes of clinically stable POAG patients were enrolled in this pilot study. Results: IOP was significantly reduced after 3 weeks of treatment. Conclusions: Gokshuradi guggul seems to exert an ocular hypotensive effect in POAG. This effect became evident after 3 weeks of therapy.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/57: Clinical efficacy of Anjana Varti and Vidalaka in Kaphaja abhishyanda w.s.r. to allergic conjunctivitis

Kirti Kumar Akhand, Babban Prasad, Pankaj Kundal

Department of Shalakya Tantra, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: kirti.akhand12@gmail.com

Background: Kaphaja Abhishyanda where itching and grittiness of the eye, foreign body sensation of eyes, watering, congestion, eye lid oedema, scanty and ropy discharge from eyes, heaviness of eyes, etc. It is quite similar to allergic conjunctivitis is a common ocular ailment of childhood and adolescent age group as described in the modern ophthalmology text. Seasonal allergic conjunctivitis and perennial allergic conjunctivitis accounts for 66–90% of all cases of allergic eye diseases. Objective: To assess the efficacy of Anjana varti and Vidalak in the management of Kaphaja Abhisyanda w.s.r. Allergic conjunctivitis. Materials and Methods: A total of 45 patients (age of 15–70 years) were selected randomly 15 in each Group A, B and C. The selection of patients was done from out-patient department/ in-patient department of Shalakya Tantra and Shreedhareeyam eye unit, N.I.A. Jaipur. Group A, patients were administered Anjana Varti/Aschyotana for 15 days. Group B, patients were administered Anjana Varti/Aschyotana and Vidalaka for 15 days. Group C, patients were administered rose water for 15 days. Results: Group A showed statistically highly significant improvement (P < 0.001) in I, Lacrimation, Burning sensation, heaviness of eye, oedema of eye lids and conjuctival congestion. Group B showed statistically highly significant improvement (P < 0.001) in I, lacrimation, heaviness of eye, edema of eyelids and the conjuctival congestion, whereas Group C showed statistically insignificant improvement in these symptoms. Conclusion: It was concluded that Anjana Varti/Aschyotana and Vidalaka were very effective in Kaphaja Abhishyanda w.s.r. allergic conjunctivitis, while Group C showed no significant response.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/58: Standardization of Khadir chhal churna and its conversion in modern tooth paste formulation and evaluation of its antimicrobial activity

Abhimanyu V Patankar, Amit C. Firke, Sheela Pargunde, Leena Nandeshwar, V. V. Gurale1, S. R. Kulkarni¹

Department of Rasa Shastra and Baishajya Kalpana, YMT Ayurvedic Medical College, Kharghar, Navi Mumbai, ¹Department of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Santacruz, Mumbai, Maharashtra, India.

E-mail: patankarabhimanyu63@gmail.com

Introduction: Dant rog, mukhrog samprapti and chikitsa have been described in Laghutrayi and Brihatrayi. The formulations used for the chikitsa are mainly churna for gharshana and kwath for kawal. Churnakalpana is a super fine pounded dry herb, which is again sieved through cloth and Pratisaran Churna is to be rubbed over teeth in oral cavity. Objective: In the current study, this is sincere effort to make the toothpaste formulation of Khadir (acacia catechu) chhal churna. As the herb Khadir has been advised to use in many of the mukha-rogas, here we evaluate the krumighna guna of khadir by in vitro anti-microbial activity against Streptococcus mutans. Materials and Methods: Paste was prepared by using the heat to soften the base prior to incorporating the solids, which have been comminuted. The paste was subjected for in vitro anti-microbial activity against S. mutans and chlorhexidine were used as standard (200 µg). Results: Toothpaste shows zone of inhibition of 2 cm and 2.5 cm at dose of 50 mg and 100 mg respectively. Standard chlorhexidine shows zone of inhibition 1.5 cm at the dose of (200 µg). Conclusion: Hence, this study revealed that the modern toothpaste formulation had good antimicrobial activity.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/59: Role of Apamarga Pratisarneeya Kshara in the management of rectal prolapse: A case study

Aditya Kumar Shil, P. Hemantha Kumar, B. Swapna

Department of Shalya Tantra, National Institute of Ayurveda, Jaipur, Rajasthan, India

E-mail: adipbt27@gmail.com

Background: Kshara karma is the para-surgical procedure described in Ayurvedic classics. As the surgery is an ultimate treatment in rectal prolapse with its own limitations. Kshara Karma can be recommended as an emerging alternative to surgery with minimal postprocedure complications. Objective: To develop a cost-effective, conducive para-surgical procedure in the management of rectal prolapse. Materials and Methods: This study was conducted on a female patient aged 48 years who was diagnosed as complete rectal prolapse, admitted in Shalya Tantra in-patient department of our institute. Procedure was performed under local anesthesia. After reducing the prolapsed rectum the Apamarga Pratisarneeya Kshara was applied circumferentially on the rectal mucosa by using a slit proctoscope and applicator. Kshara was applied for 2 min (100 Matrakala) till the color of rectal mucosa was changed to Pakwvajambuphalavarna, then the area was washed with distilled water, followed by Nimbuswarasa. The process was repeated 4 times to cover whole circumference of the rectal mucosa. Matravasti with Yashtimadhughrita was given twice daily for 7 days from first postprocedure day, followed by with Changerighrita twice daily for 15 days. Patient was advised pathyaapthyaaahara-vihara. Results: Patient had relief from symptoms of rectal prolapse. No rectal prolapse was complained during follow-up period. Conclusion: Kshara karma is a conducive, day care procedure and is very cost-effective, which can be performed under local anesthesia with minimal and trivial postprocedure complications which are negligible and can be recommended as an emerging alternative to surgery.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/60: Understanding congestive cardiac failure by pulse – Nadipariksha

Anil Digambar Vaidya

Ayurveda Clinic Marve, Mumbai

E-mail: drvaidya46@yahoo.com

Background: Pulse is one of the parameters for heart working. Using Nadipariksha reduction in pumping of the heart in congestive cardiac failure (CCF) can be graded from 0 to 15, where 0 indicates 100% pumping. According to gradation, we can understand the condition of the heart and thereby improvement in patient health state. Also, through this method we can differentiate CCF into left or right ventricular failure. Objectives: To prove relation between the pulse and reduction in pumping of the heart, to judge improvement in patient by pulse gradation and to correlate the pulse gradation with left ventricular ejection factor (LVEF). Materials and Methods: A total of 151 subjects of heart disease including CCF, other noncuring heart diseases and from relapse of previous illnesses like ischemic heart disease were studied on out-patient department basis for last 20 years. Improvement in CCF patients was judged by gradation, which correlates with disappearing signs symptoms and improvement in health. Patients with noncuring diseases, e.g., bone fracture, giddiness, Amavat were diagnosed as CCF by their pulse and were treated with Ayurvedic medicines along with treatment of the disease. Results: The diagnosis was done by pulse gradation and echo-cardiogram. Improvement after treatment was observed in health status and as judged by pulse gradation. LVEF did not show improvement in aortic regurgitation and weakness. Conclusion: The pulse is definitely effective in diagnosing CCF. Its gradation is useful in understanding heart's power and health improvement. Pulse gradation can be of clinical significance.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/61: Effect of Painmukti MJ tablets, Painmukti cream and Painmukti-sandhical tablets in patients suffering from chronic pain (including arthritis)

Renuka Munshi, Dipti Kumbhar, Supriya Bhalerao, Jairam Jagasia¹, Ashish Parikh¹

Departments of Clinical Pharmacology and ¹Orthopedics, TNMC and BYL Nair Ch. Hospital, Mumbai, Maharashtra, India

E-mail: dipti.wadangekar@gmail.com

Background: Common chronic pain conditions include headache, low back pain and arthritic pain. Although there are many analgesics available, their chronic use can lead to adverse effects on the body. Ayurveda recommends various preparations for the management of pain. Painmukti MJ tablets contain herbs like Nirgundhi, Rasna, Shallaki and Shunthi while Painmukti-Sandhical tablets contain Asthishrunkhla, Lakha, Aswagandha, Mukta. Objective: To evaluate comparative efficacy and safety of Painmukti MJ tablets, Painmukti-cream and Painmukti-sandhical tablets in patients with chronic pain. Materials and Methods: This study was initiated after Institutional Ethics Committee approval. Patients with chronic pain lasting >3 months were randomized to one of 3 groups and received either tablet Painmukti MJ + Painmukti cream (Group I), tablet Painmukti MJ + Painmukti-sandhical tablets + Painmukti cream (Group II) and placebo tablets + diclofenac gel (Group III) for 3 months. Monthly assessments included pain relief using modified Western Ontario and McMaster Universities Arthritis Index (WOMAC), visual analog scales (VAS), Quality of life using EQ-five-dimensions questionnaire and analgesic requirement. Results: Totally 106 patients were recruited (85 with arthritis) of which 2 patients were withdrawn following adverse events. Significant improvement in pain score (WOMAC and VAS) was seen in both Groups I and II at the end of 3 months of therapy as compared to the baseline (34–21 in Group I; 33–24 in Group II and 36–26 in Group III). Quality of life improved significantly in Group I with a decreased need for analgesics. Conclusion: Thus, Painmukti and Sandhical medications, which contain a combination of herbs with analgesic and anti-inflammatory properties, possess qualities that enhance agni, pacify ama and vitiate vata dosha.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/62: Evaluation of the anti-depressant activity of the aqueous extract of Tinospora cordifolia in two experimental models of depression using behavioral and biochemical parameters

Renuka P. Munshi, Bhagyeshri N. Rane, Deepika N. Kshirsagar

Department of Clinical Pharmacology, TNMC and BYL Nair Ch. Hospital, Mumbai, Maharashtra, India

E-mail: dkkulkarni10@gmail.com

Background: Depression is referred to as the common cold of psychiatry, but it makes light impact of an illness that can steal from a person his ability to conduct and enjoy a normal life. Current treatment provides some improvement in clinical condition of patients with burden of their adverse effects hence need to explore alternatives from Indian medicinal system that are effective and safe. Objective: To explore antidepressant potential of Tinospora cordifolia (TC) by exploring its role on the neurotransmitters – serotonin, acetylcholine esterase and monoamine oxidase (MAO) enzymes. Methods: Wistar rats were evaluated for anti-depressant activity using tail suspension test (TST) and forced swimming test (FST) in separate groups. Fluoxetine: 20 mg/kg/day and test drug (crude aqueous extract of TC 400 mg/kg/day) was administered orally for 21 successive days, 1-h prior to subjecting the groups to respective behavioral tests. Blood serotonin was analyzed on weekly intervals. On day 21, animals were sacrificed and the excised brain was assessed for serotonin, acetyl-cholinesterase (ACh) activity, and MAO-A and MAO-B levels. Results: A significant decrease in immobility period (1.835 ± 0.61 and 2.8075 ± 0.39) in both models. Significant increase in blood (9.552 ± 0.366 and 12.331 ± 0.306) and brain serotonin (0.866 ± 0.021 and 0.895 ± 0.056) levels, significant diminution in brain ACh (0.0543 ± 0.003 and 0.0439 ± 0.0113); MAO-A (23.79 ± 1.06 and 26.61 ± 1.10) and MAO-B (8.33 ± 1.80 and 8.74 ± 0.89) levels was observed in TST and FST group, demonstrating anti-depressant activity. Conclusion: TC modulated altered neurotransmitter and enzyme levels towards normal demonstrating anti-depressant effect.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/63: Pharmacological evaluation of anti-inflammatory and analgesic activity of Blepharispermum subsessile DC root on albino rats

Amruta Jadhav, R. N. Acharya¹, Mukesh Nariya²

Department of Dravyaguna, DY Patil School of Ayurveda, Nerul, Navi Mumbai, Maharashtra, India, ¹Department of Dravyaguna, I.P.G.T. and R.A., Gujarat Ayurved University, ²Pharmacology lab, I.P.G.T. and R.A., Gujarat Ayurved University, Jamnagar, Gujarat, India

E-mail: dramruta113@gmail.com

Background: In Ayurveda the root of Rasna is a most frequently used herbal drug for the management of vaticdisorders (pain and inflammation). The root Blepharispermum subsessile DC (Asteraceae), known as Rasna by the traditional practitioner of Odisha and Chhattisgarh region of India, is marketed with a highest price as Rasna in this region. Until now, the drug is not evaluated for its therapeutic claim in a scientific way. Objective: To evaluate the anti-inflammatory and analgesic effect of root of B. subsessile. Materials and Methods: The decoction of B. subsessile root (4.5 ml/ kg), was evaluated on Wister strain albino rats, for its anti-inflammatory (carrageenan and formalin-induced paw edema) and Analgesic activity (tail flick and formalin-induced paw licking method). Results: Decoction of B. subsessile inhibited both the phases of carrageenan-induced inflammatory responses significantly compared to the control group. However, in formalin-induced paw edema model the drug showed moderate anti-inflammatory response. In the formalin-induced paw licking both test drugs enhanced the latency of onset of paw licking as well as decrease in number of formalin-induced paw licking. Though the result found is not statistically significant but the percentage wise decrease in number of paw licking is suggestive that the test drugs have mild inhibitory activity against formalin-induced inflammatory pain while the drug is having minimal effect against radiant heat-induced pain in rats compared to control group. Conclusion: DC B. subsessile root possesses significant anti-inflammatory and mild analgesic activity while tested on Wister strain albino rats.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/64: Effect of Ashwagandharishta on learning and memory in experimental models of scopolamine induced amnesia

S. Shirolkar, S. B. Raut, Rao Rajendra Prasad, P. A. Marathe, N. Rege Nirmala

Department of Pharmacology and Therapeutics, Seth GS Medical College and KEM Hospital, Mumbai, Maharashtra, India

E-mail: rajendra.rajen74@gmail.com

Background: Ashwagandharishta (ASH), a polyherbal formulation is recommended in Ayurveda for loss of memory, weight loss, fainting, headache, psychosis, senile debility. Effects of ASH on memory have not been studied before. Objective: To evaluate the effect of ASH on learning and memory using scopolamine-induced memory impairment in animals. Materials and Methods: Following Animal Ethics Committee permission, 18 Wistar rats and 18 Swiss albino mice were used in two behavioral paradigms; Morris water maze (MWM) and elevated plus maze (EPM) respectively. Each experimental model had three groups of 6 animals each such as distilled water control, rivastigmine (2.4 mg/kg/day) as positive control and ASH (0.13 ml/kg to mice and 4.9 ml/kg to rats). All drugs were administered orally for 15 days. Scopolamine was given to all animals in the dose of 3 mg/kg I.P. to induce amnesia 90 min after the last dose on 15^th day. Training was started 45 min after scopolamine administration in MWM for rats and EPM for mice. Twenty-four hours later, memory of the animals was assessed using escape latency (MWM) and transfer latency (EPM). Data was analyzed using ANOVA with Tukey's post-hoc test (P < 0.05). Results: ASH showed statistically significant results as compared to vehicle control and comparable findings to rivastigmine in both the experiments (P < 0.05). Conclusion: Administration of ASH for 15 consecutive days significantly improved memory and learning of experimental animals. ASH may be considered as a promising agent for enhancing learning and memory.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/65: Effect of systemic Ayurvedic medicines in management of branch retinal vein occlusion with chronic macular edema: A case study

Dipti Nene

Department of Shalaya Tantra, DY Patil School of Ayurveda, Navi Mumbai, Maharashtra, India.

E-mail: dipti.nene@gmail.com

Background: Branch retinal vein occlusion (BRVO) is retinal vascular disease. Arteriosclerosis and hypertension are its predisposing factors. It presents as sudden onset of painless unilateral visual field defect, metamorphopsia. It complicates into chronic macular edema (CME) leading to poor visual acuity. Though pan-retinal photocoagulation (PRP) is the treatment for CME, it is not much beneficial in chronic cases. There is a need for Ayurvedic solutions in chronic cases such as BRVO and CME. Objectives: To explore pathogenesis in Ayurvedic terms and to find out Ayurvedic treatment for this condition. Methodology: A 59-year-old male patient (known hypertensive) came to out-patient department for diminished vision of right eye (6/60 and 6/60 with glasses), central scotoma, abnormal color vision and metamorphopsia. He had a sudden onset of these symptoms 17 months back. He was previously diagnosed as BRVO and CME and treated with topical steroids followed by intra-ocular Avastin injection twice. PRP was also done. Patient then approached for Ayurvedic treatment. After drawing suitable Ayurvedic pathogenesis (samprapti) by ashtavidha parikshan, following treatment was given: Oral pittashaman chikitsa and anuloman dravya and local bidalak (application of herbal paste over eyelid) for 3 months. Results: The distortion of images (metamorphopsia) was significantly reduced as recorded by Amsler grid. Before treatment visual acuity of affected (right) eye was 6/60 and 6/60 with glasses. After treatment visual acuity of affected eye was 6/18 and 6/9 with glasses. There was an improvement in near vision and color vision. Conclusion: Systematic Ayurvedic treatment is effective in BRVO with CME.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S5–S25.

UA/OP/66: Clinical study of psoriasis research

Vikram Pandit

Aparna Clinic, Solapur, Maharashtra, India

E-mail: researchvikram@yahoo.co.in Background: Psoriasis is a noncontiguous inflammatory skin diseases characterized by recurring reddish patches covered with silvery scales. Symptoms and signs of psoriasis resembles with kitibh kushtha as mentioned in Ayurveda. 8% of the world's population is suffering from psoriasis. The changes occurring in the skin due to psoriasis can be reverted by treatment with Ayurvedic medicinal plants. Objectives: To evaluate the effect of Ayurvedic drug regimen in the management of psoriasis. Materials and Methods: A total of 25 patients of psoriasis were selected for the study and individual consent was taken. Ayurvedic drug regimen consisting of Tinospora cardiofolia 500 mg bd, Withania somnifera 500 mg hs, Azadirakta indica, Neem leaves Churna 100 mg daily after lunch, external application of Neem soap to reduce itching, Shatadhauta Ghrita and Aloe vera for local application, Aloe vera preparation like kumari asava (200 ml before meal) if the liver function was abnormal was advised to the patients. The patients were also asked to listen to music especially kedar raga in the evening (6 p.m.–8 p.m.). 10 patients having low Vitamin B12 levels were advised to take 500 ml cow milk daily against treatment for Vitamin B12 deficiency and calcium deficiency. Pathya apathya was prescribed as per Charak Samhita. Hematological investigations viz. Serum IgE, serum IgM, serum IgA and routine biochemicals were done before and after treatment. Results: Ayurvedic drug regimen gave promising result in improving psoriasis. Vitamin B12 deficiency in psoriasis was resolved by Cow's milk. Improvement in ejection fraction values was observed. Also, punch biopsy was done to observe histopathological changes. There was hematological and histopathological reversion. Conclusion: Psoriasis can be treated with our Indian medicinal plants Ayurveda siddhanta and hematological reversion. Histo-pathological reversion is the final answer for this autoimmune disease.

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Update Ayurveda 2014 Abstracts: Oral Presentation

UA/OP/01: Oral delivery of Bromelain using calcium alginate beads

UA/OP/02: Analytical study to explore the pharmacokinetic aspect of Basti (Rectal drug administration)

UA/OP/03: Diosgenin ameliorates diabetes related renal injury through suppression of oxidative stress and inflammation

UA/OP/04: Evaluation of anticonvulsant activity of Pistacia integerrima in zebra fish and rodent models of epilepsy

UA/OP/05: Validation of RP-HPLC methods for estimation of Lupeol, Wedelolactone and β -Asarone from Symplocos racemosa, Eclipta Alba, Acorus calamus and their marketed Ayurvedic and herbal formulations

UA/OP/06: Macaranga peltata - Potential Wound Healing Agent

UA/OP/07: Cytochrome P450 inhibition potential of Trigonella (Fenugreek), Trachyspermum (Ajwaine) and Nigella Sativa (Kalonji) in rat liver microsomes

UA/OP/08: PCOS-Merging Ayurvedic and Allopathic Management

UA/OP/09: Protective action of Carissa Carandas in Gentamicin induced nephropathy via attenuation of oxidative stress

UA/OP/10: Saraca Indica fraction prevents cataract development and progression in Streptozotocin-induced diabetic rats: an aldose reductase inhibitory activity

UA/OP/11: Assessment of antimicrobial properties of hydro alcoholic extract of Morinda citrifolia

UA/OP/12: Anti-epileptogenic and anti oxidant effect of Borneol: assessment in pentylenetetrazole induced kindling model of epilepsy

UA/OP/13: Punica Granatum Fruit peel: A potential candidate for holistic treatment in inflammatory disorder of Intestine

UA/OP/14: Marker-based standardization of two ayurvedic formulations: Arka vati and Lavangadi vati

UA/OP/15: Hridayavarga Aushadhi-Carissa carandas: Evaluation of its antihypertensive activity

UA/OP/16: An upshayatmaka trial of Shreephaladi Vati and Marichyadi Churna in Grahani dosha

UA/OP/17: Studies on Pravala Moola and Shakha Bhasma and Pisti w.s.r. to pharmaco-kinetics

UA/OP/18: Clinical evaluation of the role of tablet cardicare and Basti treatment in the management of coronary artery disease (Hridyaroga)

UA/OP/19: Pharmacological evaluation of antipyretic and analgesic activity of Pippali (Piper longum linn.) fruit lavigated with cow's urine

UA/OP/20: A preliminary phytochemical evaluation of leaf and seed of Ipoemia pentaphylla linn

UA/OP/21: Krimi chiktsa in Amlapitta and its significance - a statistical analysis

UA/OP/22: Wound healing activity of standardized methanolic extract of Randia dometorum (rubiacae)

UA/OP/23: Gastro protective effect and biomarker-based pharmacokinetic profile of an Ayurvedic formulation: Bilvadileha

UA/OP/24: Correlation between Mamsa sarata and muscle strength

UA/OP/25: Role of modern analytical validation of Ayurveda Sidhipariksha for ayurvedic formulation w.s.r. to Bhasma form

UA/OP/26: A randomized control trial of Arjuna and Agnimantha on metabolic syndrome

UA/OP/27: Rosa damascena flower petals: A potential agent for oxidative stress related disorders

UA/OP/28: Pharmacognostic evaluation of Mimusops elengi L. flowers: An important Ayurvedic medicinal plant

UA/OP/29: Estrogenic effect of an Ayurvedic medicinal plant: Saraca asoca flowers

UA/OP/30: Evaluation of polyherbal oral formulation for antidiabetic activity

UA/OP/31: Enhancement of wound healing activity utilizing combination of napthoquinone dimer from Onosma echioides and Yashad bhasma

UP/OP/32: Effect of Pratisarniya kshara in the management of Gudabhramsha

UA/OP/33: Assessment of state of aggravated Doshas through physical and bio-chemical examination of blood: A diagnostic study

UA/OP/34: Multiple lipomatosis and its Ayurvedic management

UA/OP/35: Role of Kushthadi lepa and Guduchi churna in the management of Vicharchika

UA/OP/36: Formulation development of an anti-inflammatory herbal transdermal film

UA/OP/37: A randomized controlled trial of Apamarga (Achyranthus aspera L.) and Vyoshadi Guggulu on Sthaulya (Overweight)

UA/OP/38: A clinical study of changes in the lipid profile after administration of Amrutadhya Guggulu w.s.r. to hyperlipidemia

UA/OP/39: Virechana karma-A statistical analysis

UA/OP/40: Handshake of Ayurveda with modern science: Novel approach towards development of antimicrobial skin formulations

UA/OP/41: Development and physico-chemical evaluation of pain balm ointment by Gunja seeds

UA/OP/42: Treadmill stress test as a measure of Deha bala w.s.r. to Pandu roga

UA/OP/43: Evaluation and standardization of Arogyavardhini Vati

UA/OP/44: Comparative in vitro Anthelmintic activity of two ayurvedic herbal drugs

UA/OP/45: Anti secretary and anti ulcer activity of Yashtimadhu Ghrita and Pittantak Yog- An experimental study

UA/OP/46: Concept of Oja vis-a-vis endoplasmic reticular function: an approach to decode the holistic view

UA/OP/47: Pharmacognostical evaluation of Ipomoea pentaphylla Linn

UA/OP/48: Clinical Study on the effect of Bringaraj Punarnava Ghana Vati on Uccharaktachapa with special reference to essential hypertension

UA/OP/49: Can In Silico Study Shift The Paradigms In Ayurveda Drug Research Leading To Prakruti Based Medication?

UA/OP/50: Validation of Retopareeksha vis-à-vis semen analysis

UA/OP/51: An Ayurvedic approach in the management of neurocysticercosis: A case study

UA/OP/52: A novel dosage form for enhancement of bioavailability of herbal topical formulations

UA/OP/53: Epidemiological survey of Pranvaha srotodusti w.s.r. to Vayudusti (air pollution)

UA/OP/54: Physicochemical study of different methods of Gandhaka Shodhana and evaluation of its antimicrobial property

UA/OP/55: The technical development of Bahyaswedana (external sudation) Karma w.s.r Sthanika (Kati, Greeva, Prusta, Janu and Hrud) Basti

UA/OP/56: The ocular hypotensive effect of Gokshuradi guggul in primary open angle glaucoma: A Pilot Study

UA/OP/57: Clinical efficacy of Anjana Varti and Vidalaka in Kaphaja abhishyanda w.s.r. to allergic conjunctivitis

UA/OP/58: Standardization of Khadir chhal churna and its conversion in modern tooth paste formulation and evaluation of its antimicrobial activity

UA/OP/59: Role of Apamarga Pratisarneeya Kshara in the management of rectal prolapse: A case study

UA/OP/60: Understanding congestive cardiac failure by pulse – Nadipariksha

UA/OP/61: Effect of Painmukti MJ tablets, Painmukti cream and Painmukti-sandhical tablets in patients suffering from chronic pain (including arthritis)

UA/OP/62: Evaluation of the anti-depressant activity of the aqueous extract of Tinospora cordifolia in two experimental models of depression using behavioral and biochemical parameters

UA/OP/63: Pharmacological evaluation of anti-inflammatory and analgesic activity of Blepharispermum subsessile DC root on albino rats

UA/OP/64: Effect of Ashwagandharishta on learning and memory in experimental models of scopolamine induced amnesia

UA/OP/65: Effect of systemic Ayurvedic medicines in management of branch retinal vein occlusion with chronic macular edema: A case study

UA/OP/66: Clinical study of psoriasis research

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