Table 2.
Primers used for quantitative polymerase chain reaction assay
| Primer | Sequence | Annealing temperature (°C) | Expected product size (bp) |
|---|---|---|---|
| CC10 | [S] 5'-GGACACACCCTCCAGTTATGA-3' | 60 | 126 |
| [A] 5'-ATGATGCTTTCTCTGGGCTTT-3' | |||
| IL-33 | [S] 5'-ACAGCAAAGTGGAAGAACACAG-3' | 60 | 162 |
| [A] 5'-CCTTTTGGTGGTTTCTCTCCTA-3' | |||
| TSLP | [S] 5’-CCCAGGCTATTCGGAAACTCA-3’ | 60 | 118 |
| [A] 5'-ACGCCACAATCCTTGTAATTGTG-3' | |||
| IL-25 | [S] 5'-AAGGAGATGGTTGGTCAGAAGA-3' | 58 | 182 |
| [A] 5'-CTCCTAATCGCAAAAGAGCATC-3' | |||
| Osteopontin | [S] 5'-CAACCGAAGTTTTCACTCCAG-3' | 58 | 173 |
| [A] 5'-ATTCAACTCCTCGCTTTCCAT-3' | |||
| CCL20/MIP-3α | [S] 5'-GAATCAGAAGCAGCAAGCAAC-3' | 60 | 209 |
| [A] 5'-TTTTTACTGAGGAGACGCACAA-3' | |||
| CXCL19/MIG | [S] 5'-GTTCTTTTCCTCTTGGGCATC-3' | 60 | 108 |
| [A] 5'-GATAGTCCCTTGGTTGGTGCT-3' | |||
| CCL17/TARC | [S] 5'-GCCCCACTGAAGATGCTG-3' | 60 | 204 |
| [A] 5'-GCCCTGCACAGTTACAAAAAC-3' | |||
| CCL22/MDC | [S] 5'-TGATTACGTCCGTTACCGTCT-3' | 60 | 179 |
| [A] 5'-AGTAGGCTCTTCATTGGCTCA-3' | |||
| CXCL5/ENA-78 | [S] 5'-TTACAGACCACGCAAGGAGTT-3' | 60 | 105 |
| [A] 5'-GTTCTTCAGGGAGGCTACCAC-3' | |||
| ECP | [S] 5'-TGCCCTCATAACAGAACTCTCA-3' | 60 | 216 |
| [A] 5'-GATGGTGGTATCCAGGTGAACT-3' | |||
| MDA-5 | [S] 5'-GTTTGGCAGAAGGAAGTGTCA-3' | 60 | 213 |
| [A] 5'-CTGTAGGGAGGCAGATGATGA-3' | |||
| TLR2 | [S] 5'-ATGCTGCCATTCTCATTCTTCT-3' | 60 | 101 |
| [A] 5'-CTCCAGGTAGGTCTTGGTGTTC-3' | |||
| TLR3 | [S]5'-CGCTAAAAAGTGAAGAACTGGA-3' | 60 | 100 |
| [A]5'-TGAAAACACCCTGGAGAAAACT-3' | |||
| TLR4 | [S] 5'-CTTCTCAACCAAGAACCTGGAC-3' | 60 | 158 |
| [A] 5'-TAGAGAGGTGGCTTAGGCTCTG-3' | |||
| TLR9 | [S] 5'-CTACAACCGCATCGTCAAACT-3' | 60 | 217 |
| [A] 5'-ATTCAGCCAGGAGAGAGAACTG-3' | |||
| EGF | [S] 5'-CTCATCACTGGTTGTGGTTCAT-3' | 60 | 133 |
| [A] 5'-CATAAAACCTTCACGACACGAA-3' | |||
| MMP9 | [S] 5'-ACCACCACAACATCACCTATTG-3' | 60 | 166 |
| [A] 5'-ACACCAAACTGGATGACGATG-3' | |||
| TGF-β1 | [S] 5'-CAGCAACAATTCCTGGCGATA-3' | 57 | 136 |
| [A] 5'-AAGGCGAAAGCCCTCAATTT-3' | |||
| CD86 | [S] 5'-CAGCCTCTTCTTCTCTCAGCAG-3' | 60 | 100 |
| [A] 5'-CTCACTGGGGTCTGTGGTCT-3' | |||
| OX40L | [S] 5'-AAATGAAGAGGAGCAAGGAGTG-3' | 60 | 247 |
| [A] 5'-CTGGGAAAGCAAAATGGTAAAG-3' | |||
| ICOSL | [S] 5'-AGGTTTTGAGCGTTGAGGTTAC-3' | 60 | 165 |
| [A] 5'-GGCTGTTGTCCGTCTTATTGAT-3' | |||
| PD-L1 | [S] 5'-GAACTACCTCTGGCACATCCTC-3' | 60 | 126 |
| [A] 5'-CACATCCATCATTCTCCCTTTT-3' | |||
| CD80 | [S] 5'-TAATAAGCAAAGGGAGCACTGG-3' | 60 | 140 |
| [A] 5'-GCACAGGAGTCTGATGAACAAA-3' | |||
| GAPDH | [S] 5'-ACCCAGAAGACTGTGGATGG-3' | 61 | 201 |
| [A] 5'-TTCTAGACGGCAGGTCAGGT-3' |
CC10, Clara cell 10-kD protein; IL, interleukin; TSLP, thymic stromal lymphopoietin; CCL, CC chemokine ligand; MIP-3α, macrophage inflammatory protein-3α; CXCL, CXC chemokine ligand; MIG, monokine induced by interferon-γ; TARC, thymus and activation-regulated chemokine; MDC, macrophage-derived chemokine; ENA-78, epithelial neutrophil-activating peptide-78; ECP, eosinophilic cationic protein; MDA-5, melanoma differentiation-associated gene 5; TLR, Toll-like receptor; EGF, epidermal growth factor; MMP9, matrix metalloproteinase 9; TGF-β1, transforming growth factor-β1; OX40L, OX40 ligand; ICOSL, inducible costimulator ligand; PD-L1, programmed cell death ligand 1; GAPDH, glyceraldehydes-3-phosphate dehydrogenase