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. 2015 Feb 3;27(2):349–360. doi: 10.1105/tpc.114.135186

Figure 1.

Figure 1.

The Developmental Decline in Shoot Regenerative Capacity.

(A) and (C) Shoot regeneration of tobacco (A) and Arabidopsis (C). The leaves from plants of different ages were used as explants for regeneration assays. For tobacco, shoots were induced on MS medium supplemented with 6-BA of different concentrations. For Arabidopsis, calli were induced from explants on CIM and shoots were then regenerated on MS medium supplemented with 0.9 μM indole-3-acetic acid (an auxin) and 2-isopentenyladenine (2-IP; a cytokinin) of different concentrations. Bar = 0.5 cm.

(B) and (D) Quantitative analyses of regenerative capacity in tobacco (B) and Arabidopsis (D). The regenerative rate was represented by the number of regenerated shoots. Nine (B) or eight (D) explants were examined. Asterisks indicate significant differences from leaf #1-2 (Student’s t test, P < 0.05). Data are means ± sd of three biological replicates.

(E) Visualization of the ProTCS:GFP reporter in explants cultured on CIM or SIM. Explants derived from early or late leaves were examined (n = 10). The number of days after transfer to CIM or SIM is indicated. Bar = 400 μm.