Skip to main content
. 2015 Feb 3;27(2):349–360. doi: 10.1105/tpc.114.135186

Figure 5.

Figure 5.

miR156-Targeted SPL Regulates the Cytokinin Response.

(A) Diagrams of effector and reporter constructs for transactivation studies. The green boxes in the ProTCS:LUC construct indicate B-type ARR binding sites.

(B) Transactivation assays. N. benthamiana leaves were infiltrated with different combinations of effector and reporter. Bar = 0.5 cm.

(C) Quantitative analyses of LUC activity in N. benthamiana leaves. Pro35S:REN was used as an internal control. Quantification was performed by normalizing LUC activity to that of REN. The LUC activity in ProTCS:LUC without effector was set to 1.0. Data are means ± sd of three biological replicates. Asterisks indicate significant differences from mock sample (Student’s t test, P < 0.05).

(D) Expression of ARR5. Seven-day-old Arabidopsis seedlings were treated with DMSO (mock) or 5 μM 6-BA for 40 min. The expression of ARR5 in mock wild-type sample was set to 1.0. Data are means ± sd of three biological replicates.