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. 2015 Feb 10;27(2):448–462. doi: 10.1105/tpc.114.135343

Figure 10.

Figure 10.

3D Homology Models of PLA2 from E. californica and C. roseus.

Peptide sequences of PLA2, derived from the used cell strains as described in Methods, were modeled to the template patatin 17 from S. cardiophyllum.

(A)Tertiary structure of E. californica PLA2, with docked 10-hydroxychelerythrine (space fill representation). The catalytic dyad formed by Ser-65 and Asp-217 represents the active site of the enzyme. Residues Thr-123 and Lys-104 represent the structure with the strongest deviation from the homologous enzyme of C. roseus.

(B) Tertiary structure of C. roseus PLA2 with docked vinblastine (space fill representation). The catalytic dyad formed by Ser-69 and Asp-220 represents the active site of the enzyme. Residues Gln-108 and Leu-126 represent the moiety with the strongest deviation from the homologous enzyme of E. californica.