Figure 2.

Molecular and Biochemical Characterization of the TKox Plants.

(A) Quantitative RT-PCR of the introduced plastid TK transgene.

(B) Total extractable TK activity in TKox and wild-type tobacco plants. The values in (A) and (B) represent the mean of four individual plants (se ± 4) (*P > 0.05; **P < 0.01).

(C) Immunoblot analysis of protein extracts from the newest fully expanded leaf of TKox and wild-type plants. M, mesophyll; V, vein tissue extracts. Proteins (5 μg) were separated on a 12% polyacrylamide gel and blotted onto nylon membrane and probed with polyclonal antibodies against TK, DXS, and PRK proteins.