Figure 2.

VPS35B Colocalizes with Retromer-Associated Proteins to MVBs/PVCs.

(A) VPS35B-GFP colocalizes with VPS29-mRFP and the late endosome marker and VPS35A-interacting protein mCherry-RabG3F but not with the Golgi marker mCherry-SYP32 or the TGN marker VHAa1-mRFP.

(B) VPS35B localization is Wm-sensitive. Punctate structures labeled by VPS35B-GFP show dilation after 90 min of treatment with 33 μM Wm, indicating an association of VPS35B-GFP with enlarged MVBs/PVCs.

(C) VPS35B localization is BFA-insensitive. VPS35B-GFP remained in punctate structures and was distinctively separated from FM4-64-labeled BFA bodies (magenta) after 1 h of treatment with 50 μM BFA.

Confocal images were taken in root tips of 6-d-old seedlings. Bars = 5 μm.