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. 2015 Feb 10;27(2):417–431. doi: 10.1105/tpc.114.132134

Figure 2.

Figure 2.

Subcellular Localization of Gle1.

The GFP signal in the nuclear envelope is marked with arrowheads.

(A) A DNA construct encoding GFP-Gle1 under the control of the cauliflower mosaic virus 35S promoter was expressed in N. benthamiana leaves via agroinfiltration. GFP fluorescence was observed by confocal microscopy.

(B) Tobacco BY-2 cells were fixed and doubled-labeled with anti-Gle1 antibodies (red) and anti-α-tubulin antibodies (green) and stained with DAPI for confocal microscopy.

(C) GFP fluorescence in root cells of the Arabidopsis transgenic plants designated ProGle1:GFP-Gle1, which express GFP-Gle1 under the endogenous Gle1 promoter, was observed by confocal microscopy. Two independent transgenic lines (lines #13 and #21) were examined for this analysis.