Figure 1.

Identification of BES1-L in A. thaliana.

(A) Schematic overview of the transcript variants from the A. thaliana BES1 locus. Introns (lines) and exons (boxes) are shown. The peptide sequence corresponding to the bipartite NLS is highlighted with red font.

(B) Gel electrophoresis results of RT-PCR. The BES1 specific primers (RT-F and RT-R) are shown in (A). An EF1α was used as a control.

(C) The expression pattern of BES1 isoforms revealed by RT-PCR in the 9-d-old seedlings and in different tissues from adult plants.

(D) GUS staining of the pBES1(L):GUS reporter line. The pBES1(L) promoter contains the 1.56-kb region upstream of the BES1-L start codon. GUS activity was monitored in 6-d-old seedlings, 9-d-old seedlings, and siliques, inflorescences, and flowers of adult plants. Bars = 1 mm (the 1st to 4th) or 0.5 mm (the 5th).

(E) The expression of BES1 isoforms after various plant hormone treatments. Seedlings were soaked in half-strength MS liquid medium containing 5 μM eBL or 50 μM ABA for 1 h or grown on half-strength MS medium containing 100 μM GA₃ or 50 μM IAA for 9 d.

(F) The expression of BES1 isoforms after several abiotic stress treatments. Seedlings on plates were treated with heat (38°C) or cold (4°C) for 1 h or grown on half-strength MS medium with 100 and 200 mM mannitol or 50 mM NaCl for 9 d.