Figure 2.

The Phenotypes of BES1-L-GFP Transgenic Plants.

(A) Three-week-old BES1-L-GFP and BES1-S-GFP plants grown on soil. Col-0 was served as a control. The lower panel shows the seventh leaves.

(B) The 4-week-old plants shown in (A).

(C) Protein levels in corresponding plants shown in (A) and (B). Proteins were detected with anti-GFP antibody. Ponceau S-stained Rubisco large subunit serves as a loading control.

(D) The relative expression levels of some BR-responsive marker genes. CPD, DWF4, and SAUR-AC1 were detected by qRT-PCR in the corresponding plants shown in (A) and (B) (means ± sd). Three technical replicates were conducted.

(E) The phenotypes of the F1 plants of bin2-1^+/− crossed with Col-0, BES1-L-GFP, or BES1-S-GFP, respectively. Upper panels, 3-week-old plants; lower panels, 5-week-old plants. Bar = 1 cm.