Figure 3.

The Phenotypes of A. thaliana Natural Accession Oy-0 Lacking BES1-L.

(A) The DNA sequence alignment of BES1 locus from Po-0, Oy-0, and Col-0. Red letters show the deletion of G³⁴ in Oy-0. Yellow letters in blue background indicate the premature stop codon for BES1-L in Oy-0. White letters in blue show the start codon of BES1-L or BES1-S.

(B) The morphologic phenotype of the 3-week-old Po-0, Oy-0, and Col-0 ecotypes, respectively.

(C) The morphologic phenotype of the 4-week-old plants corresponding to that in (B).

(D) The phenotype of the 3-week-old transgenic Oy-0 with gBES1-GFP (corresponding to Col-0) and gBES1-ΔG-GFP (corresponding to Oy-0). Typical individuals are shown. Bar = 1 cm.

(E) Protein gel blotting detection of BES1-GFP in the rosette leaves of plants shown in (D).

(F) The flowering time of T1 gBES1-GFP/Oy-0 (n = 11) and gBES1-ΔG-GFP/Oy-0 (n = 10) transgenic plants and Col-0. Data are original data (plots) and means ± se. Student's t tests were used to determine the significant levels. ***P < 0.001 and *P < 0.05.