Figure 7.

Differential Regulation of BES1-S and BES1-L Expression.

(A) Relative expression level of BES1 determined by qRT-PCR in the wild type (En-2) and bes1-D backgrounds. Error bars indicate sd. Three technical replicates were conducted.

(B) The expression of BES1-S and BES1-L in bes1-D, bzr1-1D, bin2-3 bil1 bil2, and wild-type controls determined by RT-PCR. EF1α was used as a control.

(C) Schematic representation of the different BES1 promoter-driven GUS reporters.

(D) GUS staining of pBES1(S):GUS, pBES1(L):GUS, and pBES1(S-M):GUS transgenic plants in En-2 and bes1-D backgrounds. Seedlings were grown on half-strength MS without or with 100 nM eBL for 7 d. Bar = 1 mm.