Figure 1. 3D Schematic of pathogen capture and detection on a printed flexible plastic microchip.

(a) Magnetic beads conjugated with antibodies to capture HIV, EBV, KSHV or E. coli are mixed with a fingerprick volume of a biological sample off-chip. (b) Captured pathogens are washed 4 times with a low conducting media (glycerol) to remove residual high electrically conductive background. (c) The captured bioagents are then lysed with Triton x-100 (d) The pathogen lysate is then injected into the chip for capacitance measurement. The captured and isolated pathogens are detected through capacitance spectroscopy of the lysate on a printed flexible plastic microchip.