Table 2.

Study procedures and assessments.

	Screening	Induction TPE					Maintenance TPE
Day/month	0	1D	2D	3D	4D	5D	1M	2M	3M	4M
Informed consent	x
In/Exclusion criteria	x
Physical examination*	x	x	x	x	x	x	x	x	x	x
Neurological examination**	x					x	x	x	x	x
IFN-β BAbs***	x					x	x			x
Lab values#	x						x	x	x
IFN-β bioavailability##	x					x	x	x	x	x
TPE		x	x	x	x		x	x	x
(S)AEs assessment and reporting	x	Whenever (serious) adverse events occurs

^*ABP, HR, Resp, body temperature, ECG;

^**Neurological examination, EDSS;

^***BAbs measured with ELISA before MxA expression;

^#Hb, WBC, PLT, electrolytes, TP, coagulation (APTT, PA, fibrinogen);

^##MxA mRNA expression (before IFN-β injection and 12 hours after injection). INF-β – interferon beta; BAbs – binding antibodies; MxA – myxovirus resistance protein A; mRNA – messenger RNA; TPE – therapeutic plasma exchange.