Figure 2.

Antibody and cell mediated immune responses generated in PEG-Pam₂Cys-treated mice following challenge with 200 PFU of PR8 influenza virus. (A) Timeline of protocols used; C57BL/6 (n = 5–10) mice were inoculated via the i.n. route with saline or 20 nmol of PEG-Pam₂Cys 3 days prior to challenge with 200PFU of PR8. (B) Mice were monitored daily following viral challenge and the percentage change in body weight recorded. (C) Hemagglutination inhibiting Ab levels in sera. (D) Representative FACS plots of the NP_366-374-specific CD8⁺ T-cell response in lungs. Numbers of NP_366–374 or PA_224–236 specific cytokine secreting CD8⁺ T-cells in (E) lungs and (F) spleen. Statistical significance was determined using an unpaired Student’s t-test (*P < 0.05).