Figure 5.

PEG-Pam₂Cys treatment alone does not activate memory CD8⁺T-cells. (A) Timeline of protocol used; C57BL/6 mice were challenged with 10^4.5 PFU of X31 virus, and 2 months later, mice received either saline or 20 nmol of PEG-Pam₂Cys. After 3 days, mice were euthanized and the CD8⁺ T-cell responses assessed. (B) Total number of CD8⁺ T-cells in the lung. (C) Numbers of PA_224–236 specific CD8⁺ T-cells secreting IFN-γ, IFN-γ, and TNF-α or IFN-γ, TNF-α, and IL-2 in the lung. Results are expressed as the mean (±1 SD). Statistical significance is denoted * on the graph and was determined using an unpaired Student’s t-test (P < 0.05).