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. 2015 Jun 1;8(6):543–551. doi: 10.1242/dmm.020107

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Expression of LOX and BMP1 in epididymal adipose tissue in control and obese rats. (A) Lysyl oxidase (LOX) mRNA levels analysed by using real-time PCR of epididymal adipose tissue from control rats fed a normal chow (CT) and rats fed a high-fat diet (HFD). Results were normalised to cyclophilin expression. (B) LOX protein levels determined by western blotting of the same samples described in A. β-actin was analysed as loading control. (C) Representative images of immunostaining of LOX are presented (magnification 40×). (D) Bone morphogenetic protein-1 (BMP1) mRNA levels were quantified by using real-time PCR analyses of epididymal adipose tissue from control rats fed a normal chow (CT) and rats fed a HFD. Expression was normalised to that of cyclophilin. Graphs represent the mean±s.d. of eight animals. Scale bars: 50 µm. *P<0.05 vs control group.