Fig. 4.

RNAP pauses during antisense transcription detected by RNET-seq. (a) Scatter plot of antisense and sense transcription in the WT strain. Each dot represents a 50-bp “tail” (Fig. 2C) region of a gene. The Pearson’s correlation coefficient (r) is shown, and y = x (dotted line) represents a positive correlation. (b) Convergent transcription and pauses in the rfaH and tatD genes of WT cells. The reads mapped to plus and minus strands of genomic DNA are shown in blue and orange, respectively. The sequences for pause sites (labeled by 1–8) are shown