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. 2015 Jun 5;5:11134. doi: 10.1038/srep11134

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Copyright © 2015, Macmillan Publishers Limited

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Tissue homogenates of WBD-infected and healthy A. cervicornis were incubated with N-Hexanoyl-DL-homoserine lactone. Aquaria were then converted to closed systems and healthy test A. cervicornis were dosed with one of the four tissue homogenate/AHL combinations: Healthy + AHL, Disease + AHL, Healthy Control, and Disease Control. After dosage, the experiment was checked every 12 hours and the health status of each coral fragment was recorded. We equated total tissue loss with coral mortality. Time to coral mortality was analysed with a two-way ANOVA that considered disease state of the dosed tissue homogenate and addition or lack of AHL as fixed effects. Standard error bars are shown.