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. 2015 May 15;128(10):1901–1911. doi: 10.1242/jcs.161703

Fig. 6.

Fig. 6.

Traction-force dynamics in migrating cells reveals that the nucleus is a propagator of internal force. (A) DIC images of migrating cells before and after trailing-edge detachment events. Spontaneous tail detachment occurs more rapidly in wild-type (WT) and EGFP-expressing cells (EGFP) than in KASH4-expressing (KASH4) cells. Scale bars: 10 µm. (B) Time-dependent profiles of decaying nuclear bisector tension (NBT) on two substrates of differing stiffness (top and bottom panels) following spontaneous detachment of the trailing edge in wild-type (WT), EGFP-expressing (EGFP) and KASH4-expressing (KASH4) cells. The drop in NBT was slower and smaller in KASH4-expressing cells compared with that of control cells. (C) Comparison of the percentage drop in NBT following spontaneous tail detachment between wild-type (WT), EGFP-expressing (EGFP) and KASH4-expressing (KASH4) cells on two substrates of differing stiffness. A larger drop is observed in control than in KASH4-expressing cells. The trend does not depend on the stiffness of the underlying substrate. Error bars represent s.e.m. N=5. **P<0.025, ***P<0.005.