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FLIM images and lifetime distribution of GUVs of different compositions. (A) FLIM images of DOPC (Ld) and SM/Chol (2:1) (Lo) GUVs (top). FLIM image of GUVs with SM/DOPC/Chol (1:1:0.7) composition (Lo+Ld) (bottom). For each pixel, the lifetime value is coded using the color scale given at the bottom. The scale bar is 10 μm. (B) Distribution of lifetime values for the three types of GUVs. To see this figure in color, go online.
