Skip to main content
. Author manuscript; available in PMC: 2016 Mar 27.
Published in final edited form as: J Mol Biol. 2015 Jan 28;427(6 0 0):1335–1344. doi: 10.1016/j.jmb.2015.01.011

Fig. 2.

Fig. 2

Changes in energy transfer in the Na+/K+-ATPase with ouabain bound. (a) Sensitized emissions of the pump construct 324LBT(αM3–M4)/T71C(β). Sensitized emission in the absence of ouabain decayed with a τSE of 1.34 ms (black trace). In the same oocyte, preincubation with ouabain slowed down the decay to 1.59 ms (gray trace). (b) Distance estimations. Distance estimated for constructs 125LBT(αM1–M2)/T71C(β) (n=13), 324LBT(αM3–M4)/T71C(β) (n=10) and 988LBT(αM9–M10)/T71C(β) (n=5) in the absence of ouabain (black) and in the presence of ouabain bound (gray). Upper bars show the Δ distances caused by ouabain bound to the pump.